Advanced

Diversity and abundance of microbial communities in uasb reactors during methane production from hydrolyzed wheat straw and lucerne

Liu, Tong ; Schnürer, Anna ; Björkmalm, Johanna LU ; Willquist, Karin LU and Kreuger, Emma LU (2020) In Microorganisms 8(9).
Abstract

The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and... (More)

The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ADM1, Methane production, Microbial community, Next-generation amplicon sequencing, Process acidification, UASB, VFA, Wheat straw hydrolysate
in
Microorganisms
volume
8
issue
9
article number
1394
pages
27 pages
publisher
MDPI AG
external identifiers
  • scopus:85091379987
  • pmid:32932830
ISSN
2076-2607
DOI
10.3390/microorganisms8091394
language
English
LU publication?
yes
id
e0da8273-1c16-4275-b195-28c588f3dd4b
date added to LUP
2020-10-27 13:37:52
date last changed
2021-03-03 02:31:41
@article{e0da8273-1c16-4275-b195-28c588f3dd4b,
  abstract     = {<p>The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance.</p>},
  author       = {Liu, Tong and Schnürer, Anna and Björkmalm, Johanna and Willquist, Karin and Kreuger, Emma},
  issn         = {2076-2607},
  language     = {eng},
  number       = {9},
  publisher    = {MDPI AG},
  series       = {Microorganisms},
  title        = {Diversity and abundance of microbial communities in uasb reactors during methane production from hydrolyzed wheat straw and lucerne},
  url          = {http://dx.doi.org/10.3390/microorganisms8091394},
  doi          = {10.3390/microorganisms8091394},
  volume       = {8},
  year         = {2020},
}