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Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase

Stoica, Leonard LU ; Ludwig, R; Haltrich, D and Gorton, Lo LU (2006) In Analytical Chemistry1949-01-01+01:00 78(2). p.393-398
Abstract
The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm(2)) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL(.)min(-1)). The P.... (More)
The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm(2)) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL(.)min(-1)). The P. sordida CDH-based lactose biosensor, proved to be the better one, has a detection limit for lactose of 1 mu M, a sensitivity of 1100 mu A(.)mM(-1.)cm(-2), a response time of 4 s (the time required to obtain the maximum peak current), and a linear range from 1 to 100 mu M lactose (correlation coefficient 0.998). The simplicity of construction and analytical characteristics make this CDH-based lactose biosensor an excellent alternative to previous lactose biosensors reported in the literature or commercially available. The CDH-lactose sensor was used to quantify the content of lactose in pasteurized milk, buttermilk, and low-lactose milk, using the standard addition method. No effects of the samples matrixes were observed. The operational stability of the sensor was tested for 11 h by continuous injection of 100 mu M lactose (290 injections). The final signal of the sensor was maintained at 98% of its initial signal, with a low standard deviation of 1.72 (RSD 2.41%). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry1949-01-01+01:00
volume
78
issue
2
pages
393 - 398
publisher
The American Chemical Society
external identifiers
  • wos:000234826400005
  • scopus:30744439064
ISSN
1520-6882
DOI
10.1021/ac050327o
language
English
LU publication?
yes
id
e0fb56c9-daa0-4216-b107-4256c4a738d6 (old id 419994)
date added to LUP
2007-10-19 14:03:12
date last changed
2019-04-10 01:45:20
@article{e0fb56c9-daa0-4216-b107-4256c4a738d6,
  abstract     = {The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm(2)) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL(.)min(-1)). The P. sordida CDH-based lactose biosensor, proved to be the better one, has a detection limit for lactose of 1 mu M, a sensitivity of 1100 mu A(.)mM(-1.)cm(-2), a response time of 4 s (the time required to obtain the maximum peak current), and a linear range from 1 to 100 mu M lactose (correlation coefficient 0.998). The simplicity of construction and analytical characteristics make this CDH-based lactose biosensor an excellent alternative to previous lactose biosensors reported in the literature or commercially available. The CDH-lactose sensor was used to quantify the content of lactose in pasteurized milk, buttermilk, and low-lactose milk, using the standard addition method. No effects of the samples matrixes were observed. The operational stability of the sensor was tested for 11 h by continuous injection of 100 mu M lactose (290 injections). The final signal of the sensor was maintained at 98% of its initial signal, with a low standard deviation of 1.72 (RSD 2.41%).},
  author       = {Stoica, Leonard and Ludwig, R and Haltrich, D and Gorton, Lo},
  issn         = {1520-6882},
  language     = {eng},
  number       = {2},
  pages        = {393--398},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry1949-01-01+01:00},
  title        = {Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase},
  url          = {http://dx.doi.org/10.1021/ac050327o},
  volume       = {78},
  year         = {2006},
}