Mitogenic effect of alpha 1-microglobulin on mouse lymphocytes. Evidence of T- and B-cell cooperation, B-cell proliferation, and a low-affinity receptor on mononuclear cells
(1990) In Scandinavian Journal of Immunology 32(1). p.37-44- Abstract
Human alpha 1-m microglobulin (alpha 1-m), a low molecular weight plasma protein, was found to exert mitogenic effects on mouse lymphocytes from lymph nodes and spleen. The stimulatory effects appeared to be strain-restricted: alpha 1-m induced a varying degree of proliferation of lymphocytes from three strains, whereas one strain responded poorly. Experiments with lymphocyte subpopulations showed only weak stimulatory effects of alpha 1-m on purified T and B lymphocytes cultivated alone. The addition of mitomycin-treated cells of the other subpopulation could not restore the proliferative responses in either T or B lymphocytes. Strong stimulations were recorded only when both T and B lymphocytes were present, indicating that the T and... (More)
Human alpha 1-m microglobulin (alpha 1-m), a low molecular weight plasma protein, was found to exert mitogenic effects on mouse lymphocytes from lymph nodes and spleen. The stimulatory effects appeared to be strain-restricted: alpha 1-m induced a varying degree of proliferation of lymphocytes from three strains, whereas one strain responded poorly. Experiments with lymphocyte subpopulations showed only weak stimulatory effects of alpha 1-m on purified T and B lymphocytes cultivated alone. The addition of mitomycin-treated cells of the other subpopulation could not restore the proliferative responses in either T or B lymphocytes. Strong stimulations were recorded only when both T and B lymphocytes were present, indicating that the T and B lymphocytes cooperate to achieve the proliferation. However, FACS studies on cultured splenocytes indicated that the proliferating cells are predominantly B lymphocytes. These data extend our earlier findings of a mitogenic effect of alpha 1-m on guinea pig lymphocytes. Furthermore, results were obtained indicating the presence of a receptor on mononuclear cells. Iodine-labelled alpha 1-m was bound to mononuclear cells prepared from spleens, and the binding could be blocked by an excess of non-labelled alpha 1-m. Scatchard plotting of the data gave an equilibrium constant of 0.7 x 10(5)/M for the binding between alpha 1-m and the receptor. Together with the documented inhibitory activity of alpha 1-m on antigen-driven proliferation of lymphocytes, these results suggest an immunoregulatory role for alpha 1-m.
(Less)
- author
- Babiker-Mohamed, H ; Akerström, B LU and Lögdberg, L
- organization
- publishing date
- 1990-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alpha-Globulins/pharmacology, Animals, B-Lymphocytes/immunology, Cell Communication, Leukocytes, Mononuclear/analysis, Lipopolysaccharides/pharmacology, Lymphocyte Activation/drug effects, Mice, Mice, Inbred Strains, Receptors, Immunologic/analysis, Species Specificity, T-Lymphocytes/immunology
- in
- Scandinavian Journal of Immunology
- volume
- 32
- issue
- 1
- pages
- 37 - 44
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:1696392
- scopus:0025289024
- ISSN
- 0300-9475
- DOI
- 10.1111/j.1365-3083.1990.tb02889.x
- language
- English
- LU publication?
- yes
- id
- e0fd6130-14ce-4e0d-b5ef-13274970b014
- date added to LUP
- 2019-05-22 10:27:53
- date last changed
- 2024-01-01 06:53:19
@article{e0fd6130-14ce-4e0d-b5ef-13274970b014,
abstract = {{<p>Human alpha 1-m microglobulin (alpha 1-m), a low molecular weight plasma protein, was found to exert mitogenic effects on mouse lymphocytes from lymph nodes and spleen. The stimulatory effects appeared to be strain-restricted: alpha 1-m induced a varying degree of proliferation of lymphocytes from three strains, whereas one strain responded poorly. Experiments with lymphocyte subpopulations showed only weak stimulatory effects of alpha 1-m on purified T and B lymphocytes cultivated alone. The addition of mitomycin-treated cells of the other subpopulation could not restore the proliferative responses in either T or B lymphocytes. Strong stimulations were recorded only when both T and B lymphocytes were present, indicating that the T and B lymphocytes cooperate to achieve the proliferation. However, FACS studies on cultured splenocytes indicated that the proliferating cells are predominantly B lymphocytes. These data extend our earlier findings of a mitogenic effect of alpha 1-m on guinea pig lymphocytes. Furthermore, results were obtained indicating the presence of a receptor on mononuclear cells. Iodine-labelled alpha 1-m was bound to mononuclear cells prepared from spleens, and the binding could be blocked by an excess of non-labelled alpha 1-m. Scatchard plotting of the data gave an equilibrium constant of 0.7 x 10(5)/M for the binding between alpha 1-m and the receptor. Together with the documented inhibitory activity of alpha 1-m on antigen-driven proliferation of lymphocytes, these results suggest an immunoregulatory role for alpha 1-m.</p>}},
author = {{Babiker-Mohamed, H and Akerström, B and Lögdberg, L}},
issn = {{0300-9475}},
keywords = {{Alpha-Globulins/pharmacology; Animals; B-Lymphocytes/immunology; Cell Communication; Leukocytes, Mononuclear/analysis; Lipopolysaccharides/pharmacology; Lymphocyte Activation/drug effects; Mice; Mice, Inbred Strains; Receptors, Immunologic/analysis; Species Specificity; T-Lymphocytes/immunology}},
language = {{eng}},
number = {{1}},
pages = {{37--44}},
publisher = {{Wiley-Blackwell}},
series = {{Scandinavian Journal of Immunology}},
title = {{Mitogenic effect of alpha 1-microglobulin on mouse lymphocytes. Evidence of T- and B-cell cooperation, B-cell proliferation, and a low-affinity receptor on mononuclear cells}},
url = {{http://dx.doi.org/10.1111/j.1365-3083.1990.tb02889.x}},
doi = {{10.1111/j.1365-3083.1990.tb02889.x}},
volume = {{32}},
year = {{1990}},
}