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Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs

Bogacz, Isabel ; Makita, Hiroki ; Simon, Philipp S. ; Zhang, Miao ; Doyle, Margaret D. ; Chatterjee, Ruchira ; Fransson, Thomas ; Weninger, Clemens LU ; Fuller, Franklin and Gee, Leland , et al. (2023) In Pure and Applied Chemistry 95(8). p.891-897
Abstract

X-ray crystallography and X-ray spectroscopy using X-ray free electron lasers plays an important role in understanding the interplay of structural changes in the protein and the chemical changes at the metal active site of metalloenzymes through their catalytic cycles. As a part of such an effort, we report here our recent development of methods for X-ray absorption spectroscopy (XAS) at XFELs to study dilute biological samples, available in limited volumes. Our prime target is Photosystem II (PS II), a multi subunit membrane protein complex, that catalyzes the light-driven water oxidation reaction at the Mn4CaO5 cluster. This is an ideal system to investigate how to control multi-electron/proton chemistry, using the flexibility of... (More)

X-ray crystallography and X-ray spectroscopy using X-ray free electron lasers plays an important role in understanding the interplay of structural changes in the protein and the chemical changes at the metal active site of metalloenzymes through their catalytic cycles. As a part of such an effort, we report here our recent development of methods for X-ray absorption spectroscopy (XAS) at XFELs to study dilute biological samples, available in limited volumes. Our prime target is Photosystem II (PS II), a multi subunit membrane protein complex, that catalyzes the light-driven water oxidation reaction at the Mn4CaO5 cluster. This is an ideal system to investigate how to control multi-electron/proton chemistry, using the flexibility of metal redox states, in coordination with the protein and the water network. We describe the method that we have developed to collect XAS data using PS II samples with a Mn concentration of <1 mM, using a drop-on-demand sample delivery method.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Manganese, photoiupac 2022, photosystem II, water oxidation, X-ray absorption spectroscopy, X-ray free electron lasers
in
Pure and Applied Chemistry
volume
95
issue
8
pages
891 - 897
publisher
IUPAC
external identifiers
  • pmid:38013689
  • scopus:85153478031
ISSN
0033-4545
DOI
10.1515/pac-2023-0213
language
English
LU publication?
yes
id
e11aa756-146b-4641-b711-6d83f263efdf
date added to LUP
2023-07-18 15:02:49
date last changed
2024-04-19 23:37:47
@article{e11aa756-146b-4641-b711-6d83f263efdf,
  abstract     = {{<p>X-ray crystallography and X-ray spectroscopy using X-ray free electron lasers plays an important role in understanding the interplay of structural changes in the protein and the chemical changes at the metal active site of metalloenzymes through their catalytic cycles. As a part of such an effort, we report here our recent development of methods for X-ray absorption spectroscopy (XAS) at XFELs to study dilute biological samples, available in limited volumes. Our prime target is Photosystem II (PS II), a multi subunit membrane protein complex, that catalyzes the light-driven water oxidation reaction at the Mn4CaO5 cluster. This is an ideal system to investigate how to control multi-electron/proton chemistry, using the flexibility of metal redox states, in coordination with the protein and the water network. We describe the method that we have developed to collect XAS data using PS II samples with a Mn concentration of &lt;1 mM, using a drop-on-demand sample delivery method.</p>}},
  author       = {{Bogacz, Isabel and Makita, Hiroki and Simon, Philipp S. and Zhang, Miao and Doyle, Margaret D. and Chatterjee, Ruchira and Fransson, Thomas and Weninger, Clemens and Fuller, Franklin and Gee, Leland and Sato, Takahiro and Seaberg, Matthew and Alonso-Mori, Roberto and Bergmann, Uwe and Yachandra, Vittal K. and Kern, Jan and Yano, Junko}},
  issn         = {{0033-4545}},
  keywords     = {{Manganese; photoiupac 2022; photosystem II; water oxidation; X-ray absorption spectroscopy; X-ray free electron lasers}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{891--897}},
  publisher    = {{IUPAC}},
  series       = {{Pure and Applied Chemistry}},
  title        = {{Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs}},
  url          = {{http://dx.doi.org/10.1515/pac-2023-0213}},
  doi          = {{10.1515/pac-2023-0213}},
  volume       = {{95}},
  year         = {{2023}},
}