Human protein HC and its IgA complex are inhibitors of neutrophil chemotaxis
Méndez, Enrique ; Fernández-Luna, Jose Luís and Grubb, A LU
(1986)
In Proceedings of the National Academy of Sciences of the United States of America
83(5).
p.1472-1475
- Abstract
Protein HC, a heterogeneously charged low molecular weight glycoprotein, and its IgA complex were isolated from human plasma and urine. Plasma from individuals with monoclonal IgA populations was used as starting material for the isolation of the protein HC-IgA complex to obtain homogeneous complex populations. Neither low molecular weight protein HC nor its IgA complex in the concentrations 30 and 600 mg/liter influenced the random migration of normal human neutrophils. The chemotactic response of neutrophils to endotoxin-activated serum was, however, attenuated in a dose-dependent way by both low molecular weight protein HC and protein HC-IgA complex. Concentrations of protein HC and its IgA complex producing significant inhibition of... (More)
Protein HC, a heterogeneously charged low molecular weight glycoprotein, and its IgA complex were isolated from human plasma and urine. Plasma from individuals with monoclonal IgA populations was used as starting material for the isolation of the protein HC-IgA complex to obtain homogeneous complex populations. Neither low molecular weight protein HC nor its IgA complex in the concentrations 30 and 600 mg/liter influenced the random migration of normal human neutrophils. The chemotactic response of neutrophils to endotoxin-activated serum was, however, attenuated in a dose-dependent way by both low molecular weight protein HC and protein HC-IgA complex. Concentrations of protein HC and its IgA complex producing significant inhibition of the chemotactic response were found to occur in plasma from healthy and diseased individuals as well as in synovial fluid from patients with rheumatoid arthritis. These results suggest that protein HC and its IgA complex play physiological roles in the regulation of the inflammatory response.
(Less)
- author
- Méndez, Enrique
; Fernández-Luna, Jose Luís
and Grubb, A
LU
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alpha-Globulins/immunology, Antibodies, Monoclonal/immunology, Antigen-Antibody Complex, Arthritis, Rheumatoid/immunology, Chemotaxis, Leukocyte, Humans, Immunoglobulin A/immunology, In Vitro Techniques, Kidney Failure, Chronic/blood, Neutrophils/immunology, Synovial Fluid/immunology
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 83
- issue
- 5
- pages
- 1472 - 1475
- publisher
- National Academy of Sciences
- external identifiers
-
- scopus:0000723272
- pmid:2419908
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.83.5.1472
- language
- English
- LU publication?
- yes
- id
- e120b42c-d456-48fb-9469-ad79db82be34
- date added to LUP
- 2021-10-26 11:30:14
- date last changed
- 2024-01-12 02:54:43
@article{e120b42c-d456-48fb-9469-ad79db82be34,
abstract = {{<p>Protein HC, a heterogeneously charged low molecular weight glycoprotein, and its IgA complex were isolated from human plasma and urine. Plasma from individuals with monoclonal IgA populations was used as starting material for the isolation of the protein HC-IgA complex to obtain homogeneous complex populations. Neither low molecular weight protein HC nor its IgA complex in the concentrations 30 and 600 mg/liter influenced the random migration of normal human neutrophils. The chemotactic response of neutrophils to endotoxin-activated serum was, however, attenuated in a dose-dependent way by both low molecular weight protein HC and protein HC-IgA complex. Concentrations of protein HC and its IgA complex producing significant inhibition of the chemotactic response were found to occur in plasma from healthy and diseased individuals as well as in synovial fluid from patients with rheumatoid arthritis. These results suggest that protein HC and its IgA complex play physiological roles in the regulation of the inflammatory response.</p>}},
author = {{Méndez, Enrique and Fernández-Luna, Jose Luís and Grubb, A}},
issn = {{0027-8424}},
keywords = {{Alpha-Globulins/immunology; Antibodies, Monoclonal/immunology; Antigen-Antibody Complex; Arthritis, Rheumatoid/immunology; Chemotaxis, Leukocyte; Humans; Immunoglobulin A/immunology; In Vitro Techniques; Kidney Failure, Chronic/blood; Neutrophils/immunology; Synovial Fluid/immunology}},
language = {{eng}},
number = {{5}},
pages = {{1472--1475}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences of the United States of America}},
title = {{Human protein HC and its IgA complex are inhibitors of neutrophil chemotaxis}},
url = {{http://dx.doi.org/10.1073/pnas.83.5.1472}},
doi = {{10.1073/pnas.83.5.1472}},
volume = {{83}},
year = {{1986}},
}