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Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width

Porras Hernández, Ana María ; Tenje, Maria LU and Antfolk, Maria LU (2022) In RSC Advances 12(46). p.30135-30144
Abstract

Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this... (More)

Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this exhibited chirality bias varied across the line width. This work serves as a guide to determine optimal micropattern width for further investigations on cell chirality bias and its prominence in e.g., disease states or upon exposure to toxic substances.

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publishing date
type
Contribution to journal
publication status
published
subject
in
RSC Advances
volume
12
issue
46
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85141936208
  • pmid:36329947
ISSN
2046-2069
DOI
10.1039/d2ra05434e
language
English
LU publication?
yes
id
e136570a-b569-46a1-b650-809212c042c5
date added to LUP
2022-12-28 14:37:49
date last changed
2024-06-14 00:36:15
@article{e136570a-b569-46a1-b650-809212c042c5,
  abstract     = {{<p>Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this exhibited chirality bias varied across the line width. This work serves as a guide to determine optimal micropattern width for further investigations on cell chirality bias and its prominence in e.g., disease states or upon exposure to toxic substances.</p>}},
  author       = {{Porras Hernández, Ana María and Tenje, Maria and Antfolk, Maria}},
  issn         = {{2046-2069}},
  language     = {{eng}},
  number       = {{46}},
  pages        = {{30135--30144}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{RSC Advances}},
  title        = {{Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width}},
  url          = {{http://dx.doi.org/10.1039/d2ra05434e}},
  doi          = {{10.1039/d2ra05434e}},
  volume       = {{12}},
  year         = {{2022}},
}