Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width
(2022) In RSC Advances 12(46). p.30135-30144- Abstract
Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this... (More)
Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this exhibited chirality bias varied across the line width. This work serves as a guide to determine optimal micropattern width for further investigations on cell chirality bias and its prominence in e.g., disease states or upon exposure to toxic substances.
(Less)
- author
- Porras Hernández, Ana María ; Tenje, Maria LU and Antfolk, Maria LU
- organization
- publishing date
- 2022-10
- type
- Contribution to journal
- publication status
- published
- subject
- in
- RSC Advances
- volume
- 12
- issue
- 46
- pages
- 10 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85141936208
- pmid:36329947
- ISSN
- 2046-2069
- DOI
- 10.1039/d2ra05434e
- language
- English
- LU publication?
- yes
- id
- e136570a-b569-46a1-b650-809212c042c5
- date added to LUP
- 2022-12-28 14:37:49
- date last changed
- 2024-07-26 05:00:22
@article{e136570a-b569-46a1-b650-809212c042c5,
abstract = {{<p>Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this exhibited chirality bias varied across the line width. This work serves as a guide to determine optimal micropattern width for further investigations on cell chirality bias and its prominence in e.g., disease states or upon exposure to toxic substances.</p>}},
author = {{Porras Hernández, Ana María and Tenje, Maria and Antfolk, Maria}},
issn = {{2046-2069}},
language = {{eng}},
number = {{46}},
pages = {{30135--30144}},
publisher = {{Royal Society of Chemistry}},
series = {{RSC Advances}},
title = {{Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width}},
url = {{http://dx.doi.org/10.1039/d2ra05434e}},
doi = {{10.1039/d2ra05434e}},
volume = {{12}},
year = {{2022}},
}