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Islet cell surface antibodies and lymphocyte antibodies in the spontaneously diabetic BB Wistar rat

Dyrberg, T ; Nakhooda, A F ; Baekkeskov, S ; Lernmark, A LU ; Poussier, P and Marliss, E B (1982) In Diabetes 31(3). p.81-278
Abstract

Plasma from 14 diabetic and 6 nondiabetic BB Wistar rats along with plasma from 6 non-BB Wistar rats was evaluated for the presence of islet cells surface antibodies (ICSA) and antibodies to spleen lymphocytes by the protein-A radioligand assay. Dispersed Wistar rat islet cells incubated with plasma from diabetic rats bound 4255 +/- 2208 cpm 125I-protein A/5 x 10(4) islet cells (mean +/- SD) compared with 984 +/- 454 cpm/5 x 10(4) islet cells in islet cells incubated with plasma from nondiabetic BB rats (P less than 0.005). Twelve of the 14 diabetic rats with a duration of diabetes for 3-11 days bound radioactivity above the mean and 2 x SC of controls. The binding of 125I-protein A did not differ between nondiabetic BB rats and non-BB... (More)

Plasma from 14 diabetic and 6 nondiabetic BB Wistar rats along with plasma from 6 non-BB Wistar rats was evaluated for the presence of islet cells surface antibodies (ICSA) and antibodies to spleen lymphocytes by the protein-A radioligand assay. Dispersed Wistar rat islet cells incubated with plasma from diabetic rats bound 4255 +/- 2208 cpm 125I-protein A/5 x 10(4) islet cells (mean +/- SD) compared with 984 +/- 454 cpm/5 x 10(4) islet cells in islet cells incubated with plasma from nondiabetic BB rats (P less than 0.005). Twelve of the 14 diabetic rats with a duration of diabetes for 3-11 days bound radioactivity above the mean and 2 x SC of controls. The binding of 125I-protein A did not differ between nondiabetic BB rats and non-BB Wistar rats. Wistar rat spleen lymphocytes incubated in diabetic plasma bound 20,249 +/- 10,783 cpm/2 x 10(6) spleen lymphocytes compared with 3460 +/- 1809 in the controls (P less than 0.005). Animals positive for ICSA correlated with those positive for spleen lymphocyte antibodies. It is concluded that ICSA and splenic lymphocyte antibodies are present in diabetic BB rats.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Autoantibodies/analysis, Diabetes Mellitus/genetics, Female, Islets of Langerhans/immunology, Lymphocytes/immunology, Male, Radioligand Assay, Rats, Rats, Inbred Strains, Receptors, Antigen, B-Cell/analysis, Rodent Diseases/genetics
in
Diabetes
volume
31
issue
3
pages
81 - 278
publisher
American Diabetes Association Inc.
external identifiers
  • scopus:0020029020
  • pmid:6759244
ISSN
0012-1797
DOI
10.2337/diab.31.3.278
language
English
LU publication?
no
id
e1d91136-0289-4d63-9ca2-a187ed309231
date added to LUP
2021-09-14 16:31:07
date last changed
2021-09-19 04:55:56
@article{e1d91136-0289-4d63-9ca2-a187ed309231,
  abstract     = {<p>Plasma from 14 diabetic and 6 nondiabetic BB Wistar rats along with plasma from 6 non-BB Wistar rats was evaluated for the presence of islet cells surface antibodies (ICSA) and antibodies to spleen lymphocytes by the protein-A radioligand assay. Dispersed Wistar rat islet cells incubated with plasma from diabetic rats bound 4255 +/- 2208 cpm 125I-protein A/5 x 10(4) islet cells (mean +/- SD) compared with 984 +/- 454 cpm/5 x 10(4) islet cells in islet cells incubated with plasma from nondiabetic BB rats (P less than 0.005). Twelve of the 14 diabetic rats with a duration of diabetes for 3-11 days bound radioactivity above the mean and 2 x SC of controls. The binding of 125I-protein A did not differ between nondiabetic BB rats and non-BB Wistar rats. Wistar rat spleen lymphocytes incubated in diabetic plasma bound 20,249 +/- 10,783 cpm/2 x 10(6) spleen lymphocytes compared with 3460 +/- 1809 in the controls (P less than 0.005). Animals positive for ICSA correlated with those positive for spleen lymphocyte antibodies. It is concluded that ICSA and splenic lymphocyte antibodies are present in diabetic BB rats.</p>},
  author       = {Dyrberg, T and Nakhooda, A F and Baekkeskov, S and Lernmark, A and Poussier, P and Marliss, E B},
  issn         = {0012-1797},
  language     = {eng},
  number       = {3},
  pages        = {81--278},
  publisher    = {American Diabetes Association Inc.},
  series       = {Diabetes},
  title        = {Islet cell surface antibodies and lymphocyte antibodies in the spontaneously diabetic BB Wistar rat},
  url          = {http://dx.doi.org/10.2337/diab.31.3.278},
  doi          = {10.2337/diab.31.3.278},
  volume       = {31},
  year         = {1982},
}