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Secreted leukocyte protease inhibitor is present in aqueous humours from cataracts and other eye pathologies.

Janciauskiene, Sabina LU ; Brandt, Lars LU ; Wallmark, Anders LU ; Westin, Ulla LU and Krakau, Torsten LU (2006) In Experimental Eye Research 82(3). p.505-511
Abstract
Previous studies identified serine, cysteine and metalloproteases in normal aqueous humours (AH) and suggested that a balance between proteases and their inhibitors may play a role in the modulation of the AH outflow. We aimed to determine whether secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, is present in AH of patients with cataract and other eye pathologies. AH was collected from 117 cataract patients of which 55 were diagnosed with more when one eye disease: cataract only (n = 62), pseudoexfoliation (PEX) (n = 26), glaucoma (n = 6), diabetes retinopathy (n = 4), iritis-uveitis (n = 4) and macular degeneration (n = 28). The total protein in AH was determined by a Bradford assay and SLPI was analyzed by... (More)
Previous studies identified serine, cysteine and metalloproteases in normal aqueous humours (AH) and suggested that a balance between proteases and their inhibitors may play a role in the modulation of the AH outflow. We aimed to determine whether secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, is present in AH of patients with cataract and other eye pathologies. AH was collected from 117 cataract patients of which 55 were diagnosed with more when one eye disease: cataract only (n = 62), pseudoexfoliation (PEX) (n = 26), glaucoma (n = 6), diabetes retinopathy (n = 4), iritis-uveitis (n = 4) and macular degeneration (n = 28). The total protein in AH was determined by a Bradford assay and SLPI was analyzed by Western blot and ELISA methods. The average concentration of total protein and SLPI in AH samples was 160 +/- 15 mu g/ml (n = 117, +/- SEM) and 500 +/- 94 pg/ml (n = 105), respectively. The cataract patients with additional eye disease(s) showed higher protein levels (201 + 35 mu g/ml) than cataract (controls) (128 31 pg/ml), P < 0.01. It is noteworthy that no correlation was found between SLPI and the total protein concentrations in AH, but SLPI was positively correlated with age (r = 0.2, P < 0.05). No statistical difference in SLPI levels was found between controls (cataract) and other pathologies, while patients with iritis/uveitis had higher SLPI levels compared to those with diabetes (P < 0.05). We show here for the first time that SLPI is present in AH and may play a role as well as serve as a marker in pathological states. (c) 2005 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
inhibitors, eye, protease, aqueous humours, secretory leukocyte protease inhibitor
in
Experimental Eye Research
volume
82
issue
3
pages
505 - 511
publisher
Elsevier
external identifiers
  • wos:000235824000019
  • pmid:16202405
  • scopus:31344434534
ISSN
0014-4835
DOI
10.1016/j.exer.2005.08.010
language
English
LU publication?
yes
id
e2153c5c-1770-4344-a355-56aac731d986 (old id 144886)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16202405&dopt=Abstract
date added to LUP
2007-07-03 14:05:23
date last changed
2019-01-06 06:33:23
@article{e2153c5c-1770-4344-a355-56aac731d986,
  abstract     = {Previous studies identified serine, cysteine and metalloproteases in normal aqueous humours (AH) and suggested that a balance between proteases and their inhibitors may play a role in the modulation of the AH outflow. We aimed to determine whether secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, is present in AH of patients with cataract and other eye pathologies. AH was collected from 117 cataract patients of which 55 were diagnosed with more when one eye disease: cataract only (n = 62), pseudoexfoliation (PEX) (n = 26), glaucoma (n = 6), diabetes retinopathy (n = 4), iritis-uveitis (n = 4) and macular degeneration (n = 28). The total protein in AH was determined by a Bradford assay and SLPI was analyzed by Western blot and ELISA methods. The average concentration of total protein and SLPI in AH samples was 160 +/- 15 mu g/ml (n = 117, +/- SEM) and 500 +/- 94 pg/ml (n = 105), respectively. The cataract patients with additional eye disease(s) showed higher protein levels (201 + 35 mu g/ml) than cataract (controls) (128 31 pg/ml), P &lt; 0.01. It is noteworthy that no correlation was found between SLPI and the total protein concentrations in AH, but SLPI was positively correlated with age (r = 0.2, P &lt; 0.05). No statistical difference in SLPI levels was found between controls (cataract) and other pathologies, while patients with iritis/uveitis had higher SLPI levels compared to those with diabetes (P &lt; 0.05). We show here for the first time that SLPI is present in AH and may play a role as well as serve as a marker in pathological states. (c) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Janciauskiene, Sabina and Brandt, Lars and Wallmark, Anders and Westin, Ulla and Krakau, Torsten},
  issn         = {0014-4835},
  keyword      = {inhibitors,eye,protease,aqueous humours,secretory leukocyte protease inhibitor},
  language     = {eng},
  number       = {3},
  pages        = {505--511},
  publisher    = {Elsevier},
  series       = {Experimental Eye Research},
  title        = {Secreted leukocyte protease inhibitor is present in aqueous humours from cataracts and other eye pathologies.},
  url          = {http://dx.doi.org/10.1016/j.exer.2005.08.010},
  volume       = {82},
  year         = {2006},
}