A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons
(2014) In Biomaterials 35(5). p.8-1420- Abstract
The recent progress in stem cell techniques has broadened the horizon for in vitro disease modeling. For desired in vivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce in vivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed... (More)
The recent progress in stem cell techniques has broadened the horizon for in vitro disease modeling. For desired in vivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce in vivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed clear effects on F-actin associated proteins, connected to the disease processes. We propose that the 3D in vitro model has higher resemblance to the AD pathology than conventional 2D cultures and could be used in further studies of the disease.
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- author
- Zhang, Dawei ; Pekkanen-Mattila, Mari ; Shahsavani, Mansoureh ; Falk, Anna LU ; Teixeira, Ana I and Herland, Anna
- publishing date
- 2014-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alzheimer Disease/enzymology, Amyloid beta-Peptides/metabolism, Cell Differentiation, Cells, Cultured, Fluorescent Antibody Technique, Humans, Induced Pluripotent Stem Cells/cytology, Models, Biological, Neurons/cytology, Real-Time Polymerase Chain Reaction, p21-Activated Kinases/metabolism
- in
- Biomaterials
- volume
- 35
- issue
- 5
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:24290439
- scopus:84890173885
- ISSN
- 1878-5905
- DOI
- 10.1016/j.biomaterials.2013.11.028
- language
- English
- LU publication?
- no
- id
- e2452b20-74df-4b32-a2c1-77d06c5058fb
- date added to LUP
- 2021-08-10 13:18:17
- date last changed
- 2024-04-20 09:39:54
@article{e2452b20-74df-4b32-a2c1-77d06c5058fb,
abstract = {{<p>The recent progress in stem cell techniques has broadened the horizon for in vitro disease modeling. For desired in vivo like phenotypes, not only correct cell type specification will be critical, the microenvironmental context will be essential to achieve relevant responses. We demonstrate how a three dimensional (3D) culture of stem cell derived neurons can induce in vivo like responses related to Alzheimer's disease, not recapitulated with conventional 2D cultures. To acquire a neural population of cells we differentiated neurons from neuroepithelial stem cells, derived from induced pluripotent stem cells. p21-activated kinase mediated sensing of Aβ oligomers was only possible in the 3D environment. Further, the 3D phenotype showed clear effects on F-actin associated proteins, connected to the disease processes. We propose that the 3D in vitro model has higher resemblance to the AD pathology than conventional 2D cultures and could be used in further studies of the disease.</p>}},
author = {{Zhang, Dawei and Pekkanen-Mattila, Mari and Shahsavani, Mansoureh and Falk, Anna and Teixeira, Ana I and Herland, Anna}},
issn = {{1878-5905}},
keywords = {{Alzheimer Disease/enzymology; Amyloid beta-Peptides/metabolism; Cell Differentiation; Cells, Cultured; Fluorescent Antibody Technique; Humans; Induced Pluripotent Stem Cells/cytology; Models, Biological; Neurons/cytology; Real-Time Polymerase Chain Reaction; p21-Activated Kinases/metabolism}},
language = {{eng}},
number = {{5}},
pages = {{8--1420}},
publisher = {{Elsevier}},
series = {{Biomaterials}},
title = {{A 3D Alzheimer's disease culture model and the induction of P21-activated kinase mediated sensing in iPSC derived neurons}},
url = {{https://lup.lub.lu.se/search/files/101076475/A_3D_Alzheimer_s_disease_culture_model.pdf}},
doi = {{10.1016/j.biomaterials.2013.11.028}},
volume = {{35}},
year = {{2014}},
}