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The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response

He, Jin-Shu; Meyer-Hermann, Michael; Xiangying, Deng; Zuan, Lim Yok; Jones, Leigh Ann; Ramakrishna, Lakshmi; de Vries, Victor C; Dolpady, Jayashree; Aina, Hoi and Joseph, Sabrina, et al. (2013) In Journal of Experimental Medicine 210(12). p.2755-2771
Abstract

The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE(+) cells in memory responses is particularly unclear. IgE(+) B cell differentiation is characterized by a transient GC phase, a bias toward the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE(+) GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B cell receptor function and increased apoptosis. IgE(+) GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are... (More)

The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE(+) cells in memory responses is particularly unclear. IgE(+) B cell differentiation is characterized by a transient GC phase, a bias toward the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE(+) GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B cell receptor function and increased apoptosis. IgE(+) GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are linked to distinct B cell differentiation fates: direct switching generates IgE(+) GC cells, whereas sequential switching gives rise to IgE(+) PCs. We propose a comprehensive model for the generation and memory of IgE responses.

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@article{e26eca08-a3ca-4011-8fcc-d8251e69779b,
  abstract     = {<p>The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE(+) cells in memory responses is particularly unclear. IgE(+) B cell differentiation is characterized by a transient GC phase, a bias toward the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE(+) GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B cell receptor function and increased apoptosis. IgE(+) GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are linked to distinct B cell differentiation fates: direct switching generates IgE(+) GC cells, whereas sequential switching gives rise to IgE(+) PCs. We propose a comprehensive model for the generation and memory of IgE responses. </p>},
  author       = {He, Jin-Shu and Meyer-Hermann, Michael and Xiangying, Deng and Zuan, Lim Yok and Jones, Leigh Ann and Ramakrishna, Lakshmi and de Vries, Victor C and Dolpady, Jayashree and Aina, Hoi and Joseph, Sabrina and Narayanan, Sriram and Subramaniam, Sharrada and Puthia, Manoj and Wong, Glenn and Xiong, Huizhong and Poidinger, Michael and Urban, Joseph F and Lafaille, Juan J and Curotto de Lafaille, Maria A},
  issn         = {1540-9538},
  keyword      = {Animals,Apoptosis,B-Lymphocytes/cytology,Cell Differentiation,Germinal Center/cytology,Green Fluorescent Proteins/genetics,Immunoglobulin Class Switching,Immunoglobulin E/metabolism,Immunoglobulin G/metabolism,Immunologic Memory,Mice,Mice, Inbred BALB C,Mice, Transgenic,Models, Immunological,Nippostrongylus,Plasma Cells/cytology,Receptors, Antigen, B-Cell/metabolism,Signal Transduction,Strongylida Infections/immunology},
  language     = {eng},
  month        = {11},
  number       = {12},
  pages        = {2755--2771},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Experimental Medicine},
  title        = {The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response},
  url          = {http://dx.doi.org/10.1084/jem.20131539},
  volume       = {210},
  year         = {2013},
}