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Relating homology between the Epstein-Barr virus BOLF1 molecule and HLA-DQw8 β chain to recent onset Type 1 (insulin-dependent) diabetes mellitus

Sairenji, T. ; Daibata, M. ; Sorli, C. H. ; Qvistbäck, H. ; Humphreys, R. E. ; Ludvigsson, J. ; Palmer, J. ; Landin-Olsson, M. LU ; Sundkvist, G. LU and Michelsen, B. , et al. (1991) In Diabetologia 34(1). p.33-39
Abstract

A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed... (More)

A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed DQw8-positive lymphocytes in an immunoblotting analysis. Epstein-Barr virus antibodies were detected at equal frequencies and similar titres in sera of 30 patients with Type 1 diabetes (16 of 30;63%) and in sera of 20 non-diabetic control subjects (13 of 20;65%). Sera from diabetic patients did not bind to DQw8 β (44-63) or BOLF1(496-515) peptides. From these data we conclude that there is no simple relationship between serological evidence of Epstein-Barr virus infection and crossreactions between homologous Epstein-Barr virus and class II major histocompatibility complex peptides.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
class II MHC molecules, EBV BOLF1, Epstein-Barr virus, molecular mimicry, Type 1 (insulin-dependent) diabetes mellitus
in
Diabetologia
volume
34
issue
1
pages
33 - 39
publisher
Springer
external identifiers
  • scopus:0026015463
  • pmid:1647336
ISSN
0012-186X
DOI
10.1007/BF00404022
language
English
LU publication?
yes
id
e29943c3-bbc3-409c-b8d0-57b488b47eff
date added to LUP
2019-09-11 09:36:26
date last changed
2024-03-13 08:00:38
@article{e29943c3-bbc3-409c-b8d0-57b488b47eff,
  abstract     = {{<p>A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed DQw8-positive lymphocytes in an immunoblotting analysis. Epstein-Barr virus antibodies were detected at equal frequencies and similar titres in sera of 30 patients with Type 1 diabetes (16 of 30;63%) and in sera of 20 non-diabetic control subjects (13 of 20;65%). Sera from diabetic patients did not bind to DQw8 β (44-63) or BOLF1(496-515) peptides. From these data we conclude that there is no simple relationship between serological evidence of Epstein-Barr virus infection and crossreactions between homologous Epstein-Barr virus and class II major histocompatibility complex peptides.</p>}},
  author       = {{Sairenji, T. and Daibata, M. and Sorli, C. H. and Qvistbäck, H. and Humphreys, R. E. and Ludvigsson, J. and Palmer, J. and Landin-Olsson, M. and Sundkvist, G. and Michelsen, B. and Lernmark, Å and Dyrberg, T.}},
  issn         = {{0012-186X}},
  keywords     = {{class II MHC molecules; EBV BOLF1; Epstein-Barr virus; molecular mimicry; Type 1 (insulin-dependent) diabetes mellitus}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{33--39}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{Relating homology between the Epstein-Barr virus BOLF1 molecule and HLA-DQw8 β chain to recent onset Type 1 (insulin-dependent) diabetes mellitus}},
  url          = {{http://dx.doi.org/10.1007/BF00404022}},
  doi          = {{10.1007/BF00404022}},
  volume       = {{34}},
  year         = {{1991}},
}