Relating homology between the Epstein-Barr virus BOLF1 molecule and HLA-DQw8 β chain to recent onset Type 1 (insulin-dependent) diabetes mellitus
(1991) In Diabetologia 34(1). p.33-39- Abstract
A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed... (More)
A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed DQw8-positive lymphocytes in an immunoblotting analysis. Epstein-Barr virus antibodies were detected at equal frequencies and similar titres in sera of 30 patients with Type 1 diabetes (16 of 30;63%) and in sera of 20 non-diabetic control subjects (13 of 20;65%). Sera from diabetic patients did not bind to DQw8 β (44-63) or BOLF1(496-515) peptides. From these data we conclude that there is no simple relationship between serological evidence of Epstein-Barr virus infection and crossreactions between homologous Epstein-Barr virus and class II major histocompatibility complex peptides.
(Less)
- author
- organization
- publishing date
- 1991-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- class II MHC molecules, EBV BOLF1, Epstein-Barr virus, molecular mimicry, Type 1 (insulin-dependent) diabetes mellitus
- in
- Diabetologia
- volume
- 34
- issue
- 1
- pages
- 33 - 39
- publisher
- Springer
- external identifiers
-
- scopus:0026015463
- pmid:1647336
- ISSN
- 0012-186X
- DOI
- 10.1007/BF00404022
- language
- English
- LU publication?
- yes
- id
- e29943c3-bbc3-409c-b8d0-57b488b47eff
- date added to LUP
- 2019-09-11 09:36:26
- date last changed
- 2024-03-13 08:00:38
@article{e29943c3-bbc3-409c-b8d0-57b488b47eff, abstract = {{<p>A role for the Epstein-Barr virus in initiating Type 1 (insulin-dependent) diabetes mellitus has been proposed since Epstein-Barr virus BOLF1(497-513) AVTPL RIFIVP PAAEY has an 11 amino acid identity with HLA-DQw8 β (49-60) AVTPL GPPAAEY. Rabbit antisera to the BOLF1 (496-515) peptide crossreacted with the homologous DQw8 β (44-63) peptide but not with the related DQw7 β(44-63) peptide, which differed from the DQw8 peptide only in an ALA to ASP substitution in position 57. Antisera to DQw8 β(49-60) reacted with the DQw8 β(44-63) peptide and BOLF1 (496-515), but not with DQw7 β (44-63). The antiserum to the BOLF1 peptide bound to denatured class II major histocompatibility complex β chains from Epstein-Barr virus-transformed DQw8-positive lymphocytes in an immunoblotting analysis. Epstein-Barr virus antibodies were detected at equal frequencies and similar titres in sera of 30 patients with Type 1 diabetes (16 of 30;63%) and in sera of 20 non-diabetic control subjects (13 of 20;65%). Sera from diabetic patients did not bind to DQw8 β (44-63) or BOLF1(496-515) peptides. From these data we conclude that there is no simple relationship between serological evidence of Epstein-Barr virus infection and crossreactions between homologous Epstein-Barr virus and class II major histocompatibility complex peptides.</p>}}, author = {{Sairenji, T. and Daibata, M. and Sorli, C. H. and Qvistbäck, H. and Humphreys, R. E. and Ludvigsson, J. and Palmer, J. and Landin-Olsson, M. and Sundkvist, G. and Michelsen, B. and Lernmark, Å and Dyrberg, T.}}, issn = {{0012-186X}}, keywords = {{class II MHC molecules; EBV BOLF1; Epstein-Barr virus; molecular mimicry; Type 1 (insulin-dependent) diabetes mellitus}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{33--39}}, publisher = {{Springer}}, series = {{Diabetologia}}, title = {{Relating homology between the Epstein-Barr virus BOLF1 molecule and HLA-DQw8 β chain to recent onset Type 1 (insulin-dependent) diabetes mellitus}}, url = {{http://dx.doi.org/10.1007/BF00404022}}, doi = {{10.1007/BF00404022}}, volume = {{34}}, year = {{1991}}, }