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Si{dotless}gi{dotless}rlarda Babesia bovis ve Babesia bigemina'ni{dotless}n reverse line blotting, nested PCR ve real time PCR teknikleri i̇le Karşi{dotless}laşti{dotless}rmali{dotless} Tani{dotless}si{dotless}

Yildirim, Alparslan ; Düzlü, Önder ; Inci, Abdullah ; Önder, Zuhal and Çiloǧlu, Arif LU (2013) In Kafkas Universitesi Veteriner Fakultesi Dergisi 19(5). p.895-902
Abstract

This study was carried out to compare Reverse Line Blotting (RLB), Nested PCR and Real Time PCR techniques in the molecular diagnosis of Babesia bovis and Babesia bigemina in cattle. Genomic DNA extractions were performed on 400 blood samples which were previously collected from cattle in various provinces of Turkey and stored in the laboratory with respect to use in different project studies. The concentrations of the DNAs were measured in Nano Drop spectrophotometer and analyzed by RLB, Nested PCR and Real Time PCR techniques after preparing the suitable concentrations. Totally 18 (4.50%), 59 (14.75%), 16 (4.00%) and 2 (0.50%) of examined samples were found to be infected with B. bovis, B. bigemina, Babesia spp. and B. bovis + B.... (More)

This study was carried out to compare Reverse Line Blotting (RLB), Nested PCR and Real Time PCR techniques in the molecular diagnosis of Babesia bovis and Babesia bigemina in cattle. Genomic DNA extractions were performed on 400 blood samples which were previously collected from cattle in various provinces of Turkey and stored in the laboratory with respect to use in different project studies. The concentrations of the DNAs were measured in Nano Drop spectrophotometer and analyzed by RLB, Nested PCR and Real Time PCR techniques after preparing the suitable concentrations. Totally 18 (4.50%), 59 (14.75%), 16 (4.00%) and 2 (0.50%) of examined samples were found to be infected with B. bovis, B. bigemina, Babesia spp. and B. bovis + B. bigemina mix, respectively by RLB. 23 (5.75%), 71 (17.75%), 7 (1.75%) and 23 (5.75%), 75 (18.75%), 9 (2.00%) of the examined samples were found to be infected with B. bovis, B. bigemina and B. bovis + B. bigemina mix by Nested PCR and Real Time PCR, respectively. When comparing the Nested PCR and RLB results with Real Time PCR assay, 94.4% and 88.8% sensitivity and both 100.0% specificity were determined, respectively. 5, 9 and 2 out of the total 16 Babesia spp. positivity's in RLB test were determined as B. bigemina, B. bovis, B. bovis + B. bigemina mix, respectively by both Real Time and Nested PCR. In conclusion, Real Time PCR was found to be more sensitive than Nested PCR and RLB in the investigation of B. bovis and B. bigemina in cattle with this study.

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alternative title
Comparative diagnosis of Babesia bovis and Babesia bigemina in cattle by reverse line blotting, nested PCR and real time PCR techniques
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Babesia bigemina, Babesia bovis, Cattle, Nested PCR, Real time PCR, Revers line blotting
in
Kafkas Universitesi Veteriner Fakultesi Dergisi
volume
19
issue
5
pages
8 pages
publisher
Kafkas University
external identifiers
  • scopus:84880965319
ISSN
1300-6045
DOI
10.9775/kvfd.2013.9072
language
Turkish
LU publication?
no
id
e29e54e8-50d2-4a4d-b56f-5062d8eff892
date added to LUP
2017-12-04 15:18:06
date last changed
2020-05-10 06:11:47
@article{e29e54e8-50d2-4a4d-b56f-5062d8eff892,
  abstract     = {<p>This study was carried out to compare Reverse Line Blotting (RLB), Nested PCR and Real Time PCR techniques in the molecular diagnosis of Babesia bovis and Babesia bigemina in cattle. Genomic DNA extractions were performed on 400 blood samples which were previously collected from cattle in various provinces of Turkey and stored in the laboratory with respect to use in different project studies. The concentrations of the DNAs were measured in Nano Drop spectrophotometer and analyzed by RLB, Nested PCR and Real Time PCR techniques after preparing the suitable concentrations. Totally 18 (4.50%), 59 (14.75%), 16 (4.00%) and 2 (0.50%) of examined samples were found to be infected with B. bovis, B. bigemina, Babesia spp. and B. bovis + B. bigemina mix, respectively by RLB. 23 (5.75%), 71 (17.75%), 7 (1.75%) and 23 (5.75%), 75 (18.75%), 9 (2.00%) of the examined samples were found to be infected with B. bovis, B. bigemina and B. bovis + B. bigemina mix by Nested PCR and Real Time PCR, respectively. When comparing the Nested PCR and RLB results with Real Time PCR assay, 94.4% and 88.8% sensitivity and both 100.0% specificity were determined, respectively. 5, 9 and 2 out of the total 16 Babesia spp. positivity's in RLB test were determined as B. bigemina, B. bovis, B. bovis + B. bigemina mix, respectively by both Real Time and Nested PCR. In conclusion, Real Time PCR was found to be more sensitive than Nested PCR and RLB in the investigation of B. bovis and B. bigemina in cattle with this study.</p>},
  author       = {Yildirim, Alparslan and Düzlü, Önder and Inci, Abdullah and Önder, Zuhal and Çiloǧlu, Arif},
  issn         = {1300-6045},
  language     = {tur},
  number       = {5},
  pages        = {895--902},
  publisher    = {Kafkas University},
  series       = {Kafkas Universitesi Veteriner Fakultesi Dergisi},
  title        = {Si{dotless}gi{dotless}rlarda Babesia bovis ve Babesia bigemina'ni{dotless}n reverse line blotting, nested PCR ve real time PCR teknikleri i̇le Karşi{dotless}laşti{dotless}rmali{dotless} Tani{dotless}si{dotless}},
  url          = {http://dx.doi.org/10.9775/kvfd.2013.9072},
  doi          = {10.9775/kvfd.2013.9072},
  volume       = {19},
  year         = {2013},
}