Cyclic l-lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation
(2022) In Biotechnology and Bioengineering 119(7). p.1903-1915- Abstract
Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic l-lactide starts from poly-condensation of l-lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic l-lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic l-lactide from l-lactic acid produced from lignocellulose has so far not been successful. The major barriers are the impurities of residual sugars and inhibitors in the crude cellulosic l-lactic acid product. Here we show a successful cyclic l-lactide synthesis from cellulosic l-lactic acid by lignocellulose biorefining with complete inhibitor... (More)
Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic l-lactide starts from poly-condensation of l-lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic l-lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic l-lactide from l-lactic acid produced from lignocellulose has so far not been successful. The major barriers are the impurities of residual sugars and inhibitors in the crude cellulosic l-lactic acid product. Here we show a successful cyclic l-lactide synthesis from cellulosic l-lactic acid by lignocellulose biorefining with complete inhibitor removal and coordinated sugars assimilation. The removal of inhibitors from lignocellulose pretreatment was accomplished by biodetoxification using a unique fungus Amorphotheca resinae ZN1. The nonglucose sugars were completely and simultaneously assimilated at the same rate with glucose by the engineered l-lactic acid bacterium Pediococcus acidilactici. The l-lactic acid production from wheat straw was comparable to that from corn starch with high optical pure (99.6%), high l-lactic acid titer (129.4 g/L), minor residual total sugars (~2.2 g/L), and inhibitors free. The cyclic l-lactide was successfully synthesized from the regularly purified l-lactic acid and verified by detailed characterizations. This study paves the technical foundation of carbon-neutral production of biodegradable PLA from lignocellulose biomass.
(Less)
- author
- He, Niling ; Jia, Jia ; Qiu, Zhongyang ; Fang, Chun ; Lidén, Gunnar LU ; Liu, Xiucai and Bao, Jie
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- biodetoxification, cellulose l-lactic acid, cyclic l-lactide, inhibitor, nonglucose sugars, Pediococcus acidilactici
- in
- Biotechnology and Bioengineering
- volume
- 119
- issue
- 7
- pages
- 1903 - 1915
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85126823250
- pmid:35274740
- ISSN
- 0006-3592
- DOI
- 10.1002/bit.28082
- language
- English
- LU publication?
- yes
- id
- e2d9fc20-ad85-4289-9052-73725b34bb51
- date added to LUP
- 2022-04-20 08:27:59
- date last changed
- 2024-04-22 03:40:04
@article{e2d9fc20-ad85-4289-9052-73725b34bb51,
abstract = {{<p>Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic l-lactide starts from poly-condensation of l-lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic l-lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic l-lactide from l-lactic acid produced from lignocellulose has so far not been successful. The major barriers are the impurities of residual sugars and inhibitors in the crude cellulosic l-lactic acid product. Here we show a successful cyclic l-lactide synthesis from cellulosic l-lactic acid by lignocellulose biorefining with complete inhibitor removal and coordinated sugars assimilation. The removal of inhibitors from lignocellulose pretreatment was accomplished by biodetoxification using a unique fungus Amorphotheca resinae ZN1. The nonglucose sugars were completely and simultaneously assimilated at the same rate with glucose by the engineered l-lactic acid bacterium Pediococcus acidilactici. The l-lactic acid production from wheat straw was comparable to that from corn starch with high optical pure (99.6%), high l-lactic acid titer (129.4 g/L), minor residual total sugars (~2.2 g/L), and inhibitors free. The cyclic l-lactide was successfully synthesized from the regularly purified l-lactic acid and verified by detailed characterizations. This study paves the technical foundation of carbon-neutral production of biodegradable PLA from lignocellulose biomass.</p>}},
author = {{He, Niling and Jia, Jia and Qiu, Zhongyang and Fang, Chun and Lidén, Gunnar and Liu, Xiucai and Bao, Jie}},
issn = {{0006-3592}},
keywords = {{biodetoxification; cellulose l-lactic acid; cyclic l-lactide; inhibitor; nonglucose sugars; Pediococcus acidilactici}},
language = {{eng}},
number = {{7}},
pages = {{1903--1915}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Cyclic l-lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation}},
url = {{http://dx.doi.org/10.1002/bit.28082}},
doi = {{10.1002/bit.28082}},
volume = {{119}},
year = {{2022}},
}