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Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain

Zelco, Aura ; Börjesson, Vanja ; De Kanter, Jurrian K. ; Lebrero-Fernandez, Cristina ; Lauschke, Volker M. ; Rocha-Ferreira, Eridan ; Nilsson, Gisela ; Nair, Syam ; Svedin, Pernilla and Bemark, Mats LU orcid , et al. (2021) In Genes and Development 35(15-16). p.1190-1207
Abstract

The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. Weidentified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they... (More)

The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. Weidentified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.

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publishing date
type
Contribution to journal
publication status
published
keywords
Meningeal leukocytes, Neonatal mouse, Preterm brain injury, Single-cell RNA sequencing
in
Genes and Development
volume
35
issue
15-16
pages
1190 - 1207
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • pmid:34301765
  • scopus:85111934220
ISSN
0890-9369
DOI
10.1101/gad.348190.120
language
English
LU publication?
no
additional info
Publisher Copyright: © 2021 Cold Spring Harbor Laboratory Press. All rights reserved.
id
e2f4732d-f837-4f97-a865-d9240a136cf9
date added to LUP
2023-12-06 17:07:04
date last changed
2024-04-19 11:44:16
@article{e2f4732d-f837-4f97-a865-d9240a136cf9,
  abstract     = {{<p>The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. Weidentified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.</p>}},
  author       = {{Zelco, Aura and Börjesson, Vanja and De Kanter, Jurrian K. and Lebrero-Fernandez, Cristina and Lauschke, Volker M. and Rocha-Ferreira, Eridan and Nilsson, Gisela and Nair, Syam and Svedin, Pernilla and Bemark, Mats and Hagberg, Henrik and Mallard, Carina and Holstege, Frank C.P. and Wang, Xiaoyang}},
  issn         = {{0890-9369}},
  keywords     = {{Meningeal leukocytes; Neonatal mouse; Preterm brain injury; Single-cell RNA sequencing}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{15-16}},
  pages        = {{1190--1207}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{Genes and Development}},
  title        = {{Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain}},
  url          = {{http://dx.doi.org/10.1101/gad.348190.120}},
  doi          = {{10.1101/gad.348190.120}},
  volume       = {{35}},
  year         = {{2021}},
}