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Regional Brain Analysis of Modified Amino Acids and Dipeptides during the Sleep/Wake Cycle

Vallianatou, Theodosia ; Bèchet, Nicholas B. LU ; Correia, Mario S.P. ; Lundgaard, Iben LU and Globisch, Daniel (2022) In Metabolites 12(1).
Abstract

Sleep is a state in which important restorative and anabolic processes occur. Understanding changes of these metabolic processes during the circadian rhythm in the brain is crucial to elucidate neurophysiological mechanisms important for sleep function. Investigation of amino acid modifications and dipeptides has recently emerged as a valuable approach in the metabolic profiling of the central nervous system. Nonetheless, very little is known about the effects of sleep on the brain levels of amino acid analogues. In the present study, we examined brain regional sleep-induced alterations selective for modified amino acids and dipeptides using UPLC-MS/MS based metabolomics. Our approach enabled the detection and identification of numerous... (More)

Sleep is a state in which important restorative and anabolic processes occur. Understanding changes of these metabolic processes during the circadian rhythm in the brain is crucial to elucidate neurophysiological mechanisms important for sleep function. Investigation of amino acid modifications and dipeptides has recently emerged as a valuable approach in the metabolic profiling of the central nervous system. Nonetheless, very little is known about the effects of sleep on the brain levels of amino acid analogues. In the present study, we examined brain regional sleep-induced alterations selective for modified amino acids and dipeptides using UPLC-MS/MS based metabolomics. Our approach enabled the detection and identification of numerous amino acid-containing metabolites in the cortex, the hippocampus, the midbrain, and the cerebellum. In particular, analogues of the aromatic amino acids phenylalanine, tyrosine and tryptophan were significantly altered during sleep in the investigated brain regions. Cortical levels of medium and long chain N-acyl glycines were higher during sleep. Regional specific changes were also detected, especially related to tyrosine analogues in the hippocampus and the cerebellum. Our findings demonstrate a strong correlation between circadian rhythms and amino acid metabolism specific for different brain regions that provide previously unknown insights in brain metabolism.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino acids, Brain regions, Dipeptides, Mass spectrometry, Metabolomics, Modifications, Sleep/wake cycle
in
Metabolites
volume
12
issue
1
article number
21
publisher
MDPI AG
external identifiers
  • pmid:35050142
  • scopus:85122770205
ISSN
2218-1989
DOI
10.3390/metabo12010021
language
English
LU publication?
yes
id
e2fd0898-6ecd-4055-877b-84dc05581de3
date added to LUP
2022-03-01 08:43:21
date last changed
2024-04-18 05:40:01
@article{e2fd0898-6ecd-4055-877b-84dc05581de3,
  abstract     = {{<p>Sleep is a state in which important restorative and anabolic processes occur. Understanding changes of these metabolic processes during the circadian rhythm in the brain is crucial to elucidate neurophysiological mechanisms important for sleep function. Investigation of amino acid modifications and dipeptides has recently emerged as a valuable approach in the metabolic profiling of the central nervous system. Nonetheless, very little is known about the effects of sleep on the brain levels of amino acid analogues. In the present study, we examined brain regional sleep-induced alterations selective for modified amino acids and dipeptides using UPLC-MS/MS based metabolomics. Our approach enabled the detection and identification of numerous amino acid-containing metabolites in the cortex, the hippocampus, the midbrain, and the cerebellum. In particular, analogues of the aromatic amino acids phenylalanine, tyrosine and tryptophan were significantly altered during sleep in the investigated brain regions. Cortical levels of medium and long chain N-acyl glycines were higher during sleep. Regional specific changes were also detected, especially related to tyrosine analogues in the hippocampus and the cerebellum. Our findings demonstrate a strong correlation between circadian rhythms and amino acid metabolism specific for different brain regions that provide previously unknown insights in brain metabolism.</p>}},
  author       = {{Vallianatou, Theodosia and Bèchet, Nicholas B. and Correia, Mario S.P. and Lundgaard, Iben and Globisch, Daniel}},
  issn         = {{2218-1989}},
  keywords     = {{Amino acids; Brain regions; Dipeptides; Mass spectrometry; Metabolomics; Modifications; Sleep/wake cycle}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Metabolites}},
  title        = {{Regional Brain Analysis of Modified Amino Acids and Dipeptides during the Sleep/Wake Cycle}},
  url          = {{http://dx.doi.org/10.3390/metabo12010021}},
  doi          = {{10.3390/metabo12010021}},
  volume       = {{12}},
  year         = {{2022}},
}