Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.
(2002) In Chromosome Research 10(1). p.21-31- Abstract
- Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for... (More)
- Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106784
- author
- Rosén, Monika LU and Edström, Jan-Erik
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animal, Chironomidae : genetics, Chromosomes : ultrastructure, DNA : genetics, Nucleic Acid Denaturation, Exodeoxyribonucleases, Oligodeoxyribonucleotides, Telomere : genetics, Support Non-U.S. Gov't, Base Sequence
- in
- Chromosome Research
- volume
- 10
- issue
- 1
- pages
- 21 - 31
- publisher
- Springer
- external identifiers
-
- wos:000173846800003
- scopus:0036164616
- ISSN
- 1573-6849
- DOI
- 10.1023/A:1014257808705
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Invertebral Developmental Biology, Udo Haecker's group (013212048)
- id
- e3c87797-6972-4c7e-ac36-a3073bcf80f6 (old id 106784)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11863067&dopt=Abstract
- date added to LUP
- 2016-04-01 12:35:29
- date last changed
- 2022-01-27 07:11:48
@article{e3c87797-6972-4c7e-ac36-a3073bcf80f6,
abstract = {{Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats.}},
author = {{Rosén, Monika and Edström, Jan-Erik}},
issn = {{1573-6849}},
keywords = {{Animal; Chironomidae : genetics; Chromosomes : ultrastructure; DNA : genetics; Nucleic Acid Denaturation; Exodeoxyribonucleases; Oligodeoxyribonucleotides; Telomere : genetics; Support Non-U.S. Gov't; Base Sequence}},
language = {{eng}},
number = {{1}},
pages = {{21--31}},
publisher = {{Springer}},
series = {{Chromosome Research}},
title = {{Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.}},
url = {{http://dx.doi.org/10.1023/A:1014257808705}},
doi = {{10.1023/A:1014257808705}},
volume = {{10}},
year = {{2002}},
}