TAP1-Deficiency Does Not Alter Atherosclerosis Development in Apoe(-/-) Mice.
Kolbus, Daniel LU ; Ljungcrantz, Irena LU ; Söderberg, Ingrid LU ; Alm, Ragnar LU ; Björkbacka, Harry LU
; Nilsson, Jan
LU
and Nordin Fredrikson, Gunilla
LU
(2012)
In PLoS ONE
7(3).
- Abstract
- Antigen presenting cells (APC) have the ability to present both extra-cellular and intra-cellular antigens via MHC class I molecules to CD8(+) T cells. The cross presentation of extra-cellular antigens is reduced in mice with deficient Antigen Peptide Transporter 1 (TAP1)-dependent MHC class I antigen presentation, and these mice are characterized by a diminished CD8(+) T cell population. We have recently reported an increased activation of CD8(+) T cells in hypercholesterolemic Apoe(-/-) mice. Therefore, this study included TAP1-deficient Apoe(-/-) mice (Apoe(-/-)Tap1(-/-)) to test the atherogenicity of CD8(+) T cells and TAP1-dependent cross presentation in a hypercholesterolemic environment. As expected the CD8(+) T cell numbers were... (More)
- Antigen presenting cells (APC) have the ability to present both extra-cellular and intra-cellular antigens via MHC class I molecules to CD8(+) T cells. The cross presentation of extra-cellular antigens is reduced in mice with deficient Antigen Peptide Transporter 1 (TAP1)-dependent MHC class I antigen presentation, and these mice are characterized by a diminished CD8(+) T cell population. We have recently reported an increased activation of CD8(+) T cells in hypercholesterolemic Apoe(-/-) mice. Therefore, this study included TAP1-deficient Apoe(-/-) mice (Apoe(-/-)Tap1(-/-)) to test the atherogenicity of CD8(+) T cells and TAP1-dependent cross presentation in a hypercholesterolemic environment. As expected the CD8(+) T cell numbers were low in Apoe(-/-)Tap1(-/-) mice in comparison to Apoe(-/-) mice, constituting ∼1% of the lymphocyte population. In spite of this there were no differences in the extent of atherosclerosis as assessed by en face Oil Red O staining of the aorta and cross-sections of the aortic root between Apoe(-/-)Tap1(-/-) and Apoe(-/-) mice. Moreover, no differences were detected in lesion infiltration of macrophages or CD3(+) T cells in Apoe(-/-)Tap1(-/-) compared to Apoe(-/-) mice. The CD3(+)CD4(+) T cell fraction was increased in Apoe(-/-)Tap1(-/-) mice, suggesting a compensation for the decreased CD8(+) T cell population. Interestingly, the fraction of CD8(+) effector memory T cells was increased but this appeared to have little impact on the atherosclerosis development.In conclusion, Apoe(-/-)Tap1(-/-) mice develop atherosclerosis equal to Apoe(-/-) mice, indicating a minor role for CD8(+) T cells and TAP1-dependent antigen presentation in the disease process. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2519755
- author
- Kolbus, Daniel
LU
; Ljungcrantz, Irena
LU
; Söderberg, Ingrid
LU
; Alm, Ragnar
LU
; Björkbacka, Harry
LU
; Nilsson, Jan
LU
and Nordin Fredrikson, Gunilla
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 7
- issue
- 3
- article number
- e33932
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- wos:000305339100084
- pmid:22479479
- scopus:84859127470
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0033932
- language
- English
- LU publication?
- yes
- id
- e4136c98-0462-4fe9-bae0-501a665be2d0 (old id 2519755)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22479479?dopt=Abstract
- date added to LUP
- 2016-04-01 14:05:55
- date last changed
- 2025-10-14 10:06:04
@article{e4136c98-0462-4fe9-bae0-501a665be2d0,
abstract = {{Antigen presenting cells (APC) have the ability to present both extra-cellular and intra-cellular antigens via MHC class I molecules to CD8(+) T cells. The cross presentation of extra-cellular antigens is reduced in mice with deficient Antigen Peptide Transporter 1 (TAP1)-dependent MHC class I antigen presentation, and these mice are characterized by a diminished CD8(+) T cell population. We have recently reported an increased activation of CD8(+) T cells in hypercholesterolemic Apoe(-/-) mice. Therefore, this study included TAP1-deficient Apoe(-/-) mice (Apoe(-/-)Tap1(-/-)) to test the atherogenicity of CD8(+) T cells and TAP1-dependent cross presentation in a hypercholesterolemic environment. As expected the CD8(+) T cell numbers were low in Apoe(-/-)Tap1(-/-) mice in comparison to Apoe(-/-) mice, constituting ∼1% of the lymphocyte population. In spite of this there were no differences in the extent of atherosclerosis as assessed by en face Oil Red O staining of the aorta and cross-sections of the aortic root between Apoe(-/-)Tap1(-/-) and Apoe(-/-) mice. Moreover, no differences were detected in lesion infiltration of macrophages or CD3(+) T cells in Apoe(-/-)Tap1(-/-) compared to Apoe(-/-) mice. The CD3(+)CD4(+) T cell fraction was increased in Apoe(-/-)Tap1(-/-) mice, suggesting a compensation for the decreased CD8(+) T cell population. Interestingly, the fraction of CD8(+) effector memory T cells was increased but this appeared to have little impact on the atherosclerosis development.In conclusion, Apoe(-/-)Tap1(-/-) mice develop atherosclerosis equal to Apoe(-/-) mice, indicating a minor role for CD8(+) T cells and TAP1-dependent antigen presentation in the disease process.}},
author = {{Kolbus, Daniel and Ljungcrantz, Irena and Söderberg, Ingrid and Alm, Ragnar and Björkbacka, Harry and Nilsson, Jan and Nordin Fredrikson, Gunilla}},
issn = {{1932-6203}},
language = {{eng}},
number = {{3}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{TAP1-Deficiency Does Not Alter Atherosclerosis Development in Apoe(-/-) Mice.}},
url = {{https://lup.lub.lu.se/search/files/3779338/2544700.pdf}},
doi = {{10.1371/journal.pone.0033932}},
volume = {{7}},
year = {{2012}},
}