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Induction of S100A9 homodimer formation in vivo

Källberg, Eva LU ; Tahvili, Sahar LU ; Ivars, Fredrik LU and Leanderson, Tomas LU (2018) In Biochemical and Biophysical Research Communications p.564-568
Abstract

We show here that increased S100A8 and S100A9 protein expression is induced in spleen of animals with active inflammation or with inoculated tumors. In tumor bearing animals an increased expression was also detected in the lung. To further analyze the induced proteins, we performed chemical cross-linking followed by Western blotting. We observed in protein extracts from spleen that both S100A8/S100A9 heterodimers as well as S100A9 homodimers were formed, both after tumor and inflammatory challenge. The cellular source for S100A9 homodimers were CD11b+GR1+ cells. S100A9 homodimers were also secreted into the extracellular space. Lastly, in the spleen from normal and tumor bearing animals cells expressing relatively... (More)

We show here that increased S100A8 and S100A9 protein expression is induced in spleen of animals with active inflammation or with inoculated tumors. In tumor bearing animals an increased expression was also detected in the lung. To further analyze the induced proteins, we performed chemical cross-linking followed by Western blotting. We observed in protein extracts from spleen that both S100A8/S100A9 heterodimers as well as S100A9 homodimers were formed, both after tumor and inflammatory challenge. The cellular source for S100A9 homodimers were CD11b+GR1+ cells. S100A9 homodimers were also secreted into the extracellular space. Lastly, in the spleen from normal and tumor bearing animals cells expressing relatively higher levels of S100A9 compared to S100A8 could be observed by immunohistochemistry. Taken together, these data show that the biologically potent dimeric form of S100A9 is induced in vivo in situations of tumor burden or inflammatory challenge.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer, Inflammation, S100A9
in
Biochemical and Biophysical Research Communications
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:85046125143
ISSN
0006-291X
DOI
10.1016/j.bbrc.2018.04.086
language
English
LU publication?
yes
id
e4aba930-bd8f-4125-977e-842e54cccacb
date added to LUP
2018-05-15 14:20:29
date last changed
2018-10-03 11:01:42
@article{e4aba930-bd8f-4125-977e-842e54cccacb,
  abstract     = {<p>We show here that increased S100A8 and S100A9 protein expression is induced in spleen of animals with active inflammation or with inoculated tumors. In tumor bearing animals an increased expression was also detected in the lung. To further analyze the induced proteins, we performed chemical cross-linking followed by Western blotting. We observed in protein extracts from spleen that both S100A8/S100A9 heterodimers as well as S100A9 homodimers were formed, both after tumor and inflammatory challenge. The cellular source for S100A9 homodimers were CD11b<sup>+</sup>GR1<sup>+</sup> cells. S100A9 homodimers were also secreted into the extracellular space. Lastly, in the spleen from normal and tumor bearing animals cells expressing relatively higher levels of S100A9 compared to S100A8 could be observed by immunohistochemistry. Taken together, these data show that the biologically potent dimeric form of S100A9 is induced in vivo in situations of tumor burden or inflammatory challenge.</p>},
  author       = {Källberg, Eva and Tahvili, Sahar and Ivars, Fredrik and Leanderson, Tomas},
  issn         = {0006-291X},
  keyword      = {Cancer,Inflammation,S100A9},
  language     = {eng},
  pages        = {564--568},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Induction of S100A9 homodimer formation in vivo},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2018.04.086},
  year         = {2018},
}