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Tuft cells : Distribution and connections with nerves and endocrine cells in mouse intestine

Cheng, Xiaowen LU ; Voss, Ulrikke LU and Ekblad, Eva LU (2018) In Experimental Cell Research 369(1). p.105-111
Abstract

Tuft cells are gastrointestinal (GI) sensory cells recognized by their characteristic shape and their microvilli “tuft”. Aims of the present study were to elucidate their regional distribution and spatial connections with satiety associated endocrine cells and nerve fibers throughout the intestinal tract. C57BL/6 J mice were used in the experiments. The small intestine was divided into five segments, and the large intestine was kept undivided. The segments were coiled into “Swiss rolls”. Numbers and topographic distribution of tuft cells and possible contacts with endocrine cells and nerve fibers were estimated in the different segments, using immunocytochemistry. Tuft cells were found throughout the intestines; the highest number was... (More)

Tuft cells are gastrointestinal (GI) sensory cells recognized by their characteristic shape and their microvilli “tuft”. Aims of the present study were to elucidate their regional distribution and spatial connections with satiety associated endocrine cells and nerve fibers throughout the intestinal tract. C57BL/6 J mice were used in the experiments. The small intestine was divided into five segments, and the large intestine was kept undivided. The segments were coiled into “Swiss rolls”. Numbers and topographic distribution of tuft cells and possible contacts with endocrine cells and nerve fibers were estimated in the different segments, using immunocytochemistry. Tuft cells were found throughout the intestines; the highest number was in proximal small intestine. Five percent of tuft cells were found in close proximity to cholecystokinin-immunoreactive (IR) endocrine cells and up to 10% were in contact with peptide YY- and glucagon-like peptide-1-IR endocrine cells. Sixty percent of tuft cells in the small intestine and 40% in the large intestine were found in contact with nerve fibers. Calcitonin gene-related peptide-IR fibers constituted one-third of the fiber-contacts in the small intestine and two-thirds in the large intestine. These observations highlight the possibility of tuft cells as modulators of GI activities in response to luminal signaling.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CCK, CGRP, Enteric nervous system, GLP-1, PYY, Tuft cells
in
Experimental Cell Research
volume
369
issue
1
pages
105 - 111
publisher
Academic Press
external identifiers
  • scopus:85047195342
  • pmid:29758188
ISSN
0014-4827
DOI
10.1016/j.yexcr.2018.05.011
language
English
LU publication?
yes
id
e4ae9f8b-4dde-4949-b3e5-eb8c7d3b8d6b
date added to LUP
2018-06-04 09:31:34
date last changed
2020-02-12 09:29:47
@article{e4ae9f8b-4dde-4949-b3e5-eb8c7d3b8d6b,
  abstract     = {<p>Tuft cells are gastrointestinal (GI) sensory cells recognized by their characteristic shape and their microvilli “tuft”. Aims of the present study were to elucidate their regional distribution and spatial connections with satiety associated endocrine cells and nerve fibers throughout the intestinal tract. C57BL/6 J mice were used in the experiments. The small intestine was divided into five segments, and the large intestine was kept undivided. The segments were coiled into “Swiss rolls”. Numbers and topographic distribution of tuft cells and possible contacts with endocrine cells and nerve fibers were estimated in the different segments, using immunocytochemistry. Tuft cells were found throughout the intestines; the highest number was in proximal small intestine. Five percent of tuft cells were found in close proximity to cholecystokinin-immunoreactive (IR) endocrine cells and up to 10% were in contact with peptide YY- and glucagon-like peptide-1-IR endocrine cells. Sixty percent of tuft cells in the small intestine and 40% in the large intestine were found in contact with nerve fibers. Calcitonin gene-related peptide-IR fibers constituted one-third of the fiber-contacts in the small intestine and two-thirds in the large intestine. These observations highlight the possibility of tuft cells as modulators of GI activities in response to luminal signaling.</p>},
  author       = {Cheng, Xiaowen and Voss, Ulrikke and Ekblad, Eva},
  issn         = {0014-4827},
  language     = {eng},
  month        = {05},
  number       = {1},
  pages        = {105--111},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Tuft cells : Distribution and connections with nerves and endocrine cells in mouse intestine},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2018.05.011},
  doi          = {10.1016/j.yexcr.2018.05.011},
  volume       = {369},
  year         = {2018},
}