Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping
(2016) In Nature Communications 7.- Abstract
Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a... (More)
Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.
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- author
- Augustsson, Per LU ; Karlsen, Jonas T ; Su, Hao-Wei ; Bruus, Henrik and Voldman, Joel
- organization
- publishing date
- 2016-05-16
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 7
- article number
- 11556
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:84968763924
- wos:000375938500001
- pmid:27180912
- ISSN
- 2041-1723
- DOI
- 10.1038/ncomms11556
- language
- English
- LU publication?
- yes
- id
- e4f59fc6-8d6f-489d-8e77-3935741bfe72
- date added to LUP
- 2016-06-22 22:27:13
- date last changed
- 2024-04-05 02:32:45
@article{e4f59fc6-8d6f-489d-8e77-3935741bfe72,
abstract = {{<p>Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.</p>}},
author = {{Augustsson, Per and Karlsen, Jonas T and Su, Hao-Wei and Bruus, Henrik and Voldman, Joel}},
issn = {{2041-1723}},
language = {{eng}},
month = {{05}},
publisher = {{Nature Publishing Group}},
series = {{Nature Communications}},
title = {{Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping}},
url = {{http://dx.doi.org/10.1038/ncomms11556}},
doi = {{10.1038/ncomms11556}},
volume = {{7}},
year = {{2016}},
}