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Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping

Augustsson, Per LU ; Karlsen, Jonas T; Su, Hao-Wei; Bruus, Henrik and Voldman, Joel (2016) In Nature Communications 7.
Abstract

Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a... (More)

Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
7
publisher
Nature Publishing Group
external identifiers
  • scopus:84968763924
  • wos:000375938500001
ISSN
2041-1723
DOI
10.1038/ncomms11556
language
English
LU publication?
yes
id
e4f59fc6-8d6f-489d-8e77-3935741bfe72
date added to LUP
2016-06-22 22:27:13
date last changed
2017-10-08 04:50:17
@article{e4f59fc6-8d6f-489d-8e77-3935741bfe72,
  abstract     = {<p>Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells' interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.</p>},
  articleno    = {11556},
  author       = {Augustsson, Per and Karlsen, Jonas T and Su, Hao-Wei and Bruus, Henrik and Voldman, Joel},
  issn         = {2041-1723},
  language     = {eng},
  month        = {05},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping},
  url          = {http://dx.doi.org/10.1038/ncomms11556},
  volume       = {7},
  year         = {2016},
}