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Regulation and function of laminin A5 during mouse and human decidualization

Yang, Zhen Shan LU orcid ; Pan, Hai Yang ; Shi, Wen Wen ; Chen, Si Ting ; Wang, Ying ; Li, Meng Yuan ; Zhang, Hai Yi ; Yang, Chen ; Liu, Ai Xia and Yang, Zeng Ming (2022) In International Journal of Molecular Sciences 23(1).
Abstract

Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by Laminin α5) is a member of the laminin family, which is mainly expressed in the basement membranes. Although laminins regulate cellular phenotype maintenance, adhe-sion, migration, growth, and differentiation, the expression, function, and regulation of laminin A5 during early pregnancy are still unknown. Therefore, we investigated the expression and role of laminin A5 during mouse and human decidualization. Laminin A5 is highly expressed in mouse decidua and artificially induced deciduoma. Laminin A5 is significantly increased under in vitro decidualization. Laminin A5 knockdown significantly inhibits the expression of... (More)

Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by Laminin α5) is a member of the laminin family, which is mainly expressed in the basement membranes. Although laminins regulate cellular phenotype maintenance, adhe-sion, migration, growth, and differentiation, the expression, function, and regulation of laminin A5 during early pregnancy are still unknown. Therefore, we investigated the expression and role of laminin A5 during mouse and human decidualization. Laminin A5 is highly expressed in mouse decidua and artificially induced deciduoma. Laminin A5 is significantly increased under in vitro decidualization. Laminin A5 knockdown significantly inhibits the expression of Prl8a2, a marker for mouse decidualization. Progesterone stimulates the expression of laminin A5 in ovariectomized mouse uterus and cultured mouse stromal cells. We also show that progesterone regulates laminin A5 through the PKA‐CREB‐C/EBPβ pathway. Laminin A5 is also highly expressed in human preg-nant decidua and cultured human endometrial stromal cells during in vitro decidualization. Lam-inin A5 knockdown by siRNA inhibits human in vitro decidualization. Collectively, our study reveals that laminin A5 may play a pivotal role during mouse and human decidualization via the PKA‐CREB‐C/EBPβ pathway.

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author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
C/EBPβ, CREB, Decidualization, Laminin A5, PKA, Uterus
in
International Journal of Molecular Sciences
volume
23
issue
1
article number
199
publisher
MDPI AG
external identifiers
  • pmid:35008625
  • scopus:85121626280
ISSN
1661-6596
DOI
10.3390/ijms23010199
language
English
LU publication?
no
additional info
Funding Information: Funding: This study was supported by the National Key Research and Development Program of China (2018YFC1004400) and National Natural Science Foundation of China (31871511 and 32171114). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
id
e4f93113-62db-499f-80dd-ee709b320ba3
date added to LUP
2024-02-28 14:55:56
date last changed
2024-04-27 13:27:23
@article{e4f93113-62db-499f-80dd-ee709b320ba3,
  abstract     = {{<p>Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by Laminin α5) is a member of the laminin family, which is mainly expressed in the basement membranes. Although laminins regulate cellular phenotype maintenance, adhe-sion, migration, growth, and differentiation, the expression, function, and regulation of laminin A5 during early pregnancy are still unknown. Therefore, we investigated the expression and role of laminin A5 during mouse and human decidualization. Laminin A5 is highly expressed in mouse decidua and artificially induced deciduoma. Laminin A5 is significantly increased under in vitro decidualization. Laminin A5 knockdown significantly inhibits the expression of Prl8a2, a marker for mouse decidualization. Progesterone stimulates the expression of laminin A5 in ovariectomized mouse uterus and cultured mouse stromal cells. We also show that progesterone regulates laminin A5 through the PKA‐CREB‐C/EBPβ pathway. Laminin A5 is also highly expressed in human preg-nant decidua and cultured human endometrial stromal cells during in vitro decidualization. Lam-inin A5 knockdown by siRNA inhibits human in vitro decidualization. Collectively, our study reveals that laminin A5 may play a pivotal role during mouse and human decidualization via the PKA‐CREB‐C/EBPβ pathway.</p>}},
  author       = {{Yang, Zhen Shan and Pan, Hai Yang and Shi, Wen Wen and Chen, Si Ting and Wang, Ying and Li, Meng Yuan and Zhang, Hai Yi and Yang, Chen and Liu, Ai Xia and Yang, Zeng Ming}},
  issn         = {{1661-6596}},
  keywords     = {{C/EBPβ; CREB; Decidualization; Laminin A5; PKA; Uterus}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Regulation and function of laminin A5 during mouse and human decidualization}},
  url          = {{http://dx.doi.org/10.3390/ijms23010199}},
  doi          = {{10.3390/ijms23010199}},
  volume       = {{23}},
  year         = {{2022}},
}