A lipid-based passivation scheme for nanofluidics
(2012) 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 p.1876-1878- Abstract
Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e5b2d2d7-e346-4e3f-8f99-704f311f4328
- author
- Fritzsche, Joachim
; Persson, Fredrik
LU
; Mir, Kalim U.
; Modesti, Mauro
; Westerlund, Fredrik
and Tegenfeldt, Jonas O.
LU
- organization
- publishing date
- 2012-01-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Lipid bilayer, Nanofluidics, Passivation, Protein-DNA interactions, Single molecules
- host publication
- Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- conference location
- Okinawa, Japan
- conference dates
- 2012-10-28 - 2012-11-01
- external identifiers
-
- scopus:84901809339
- ISBN
- 9780979806452
- language
- English
- LU publication?
- yes
- id
- e5b2d2d7-e346-4e3f-8f99-704f311f4328
- date added to LUP
- 2018-10-20 08:47:29
- date last changed
- 2023-08-07 14:01:00
@inproceedings{e5b2d2d7-e346-4e3f-8f99-704f311f4328, abstract = {{<p>Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.</p>}}, author = {{Fritzsche, Joachim and Persson, Fredrik and Mir, Kalim U. and Modesti, Mauro and Westerlund, Fredrik and Tegenfeldt, Jonas O.}}, booktitle = {{Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012}}, isbn = {{9780979806452}}, keywords = {{Lipid bilayer; Nanofluidics; Passivation; Protein-DNA interactions; Single molecules}}, language = {{eng}}, month = {{01}}, pages = {{1876--1878}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{A lipid-based passivation scheme for nanofluidics}}, year = {{2012}}, }