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A lipid-based passivation scheme for nanofluidics

Fritzsche, Joachim; Persson, Fredrik LU ; Mir, Kalim U.; Modesti, Mauro; Westerlund, Fredrik and Tegenfeldt, Jonas O. LU (2012) 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 p.1876-1878
Abstract

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Lipid bilayer, Nanofluidics, Passivation, Protein-DNA interactions, Single molecules
host publication
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
conference location
Okinawa, Japan
conference dates
2012-10-28 - 2012-11-01
external identifiers
  • scopus:84901809339
ISBN
9780979806452
language
English
LU publication?
yes
id
e5b2d2d7-e346-4e3f-8f99-704f311f4328
date added to LUP
2018-10-20 08:47:29
date last changed
2019-04-12 09:38:43
@inproceedings{e5b2d2d7-e346-4e3f-8f99-704f311f4328,
  abstract     = {<p>Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.</p>},
  author       = {Fritzsche, Joachim and Persson, Fredrik and Mir, Kalim U. and Modesti, Mauro and Westerlund, Fredrik and Tegenfeldt, Jonas O.},
  isbn         = {9780979806452},
  keyword      = {Lipid bilayer,Nanofluidics,Passivation,Protein-DNA interactions,Single molecules},
  language     = {eng},
  location     = {Okinawa, Japan},
  month        = {01},
  pages        = {1876--1878},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {A lipid-based passivation scheme for nanofluidics},
  year         = {2012},
}