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Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report

Saša, Anžej Doma; Andreas, Hillarp; Tadej, Pajič; Dušan, Andoljšek; Peter, Černelč and Irena, Preložnik Zupan (2016) In Biochemia Medica 26(2). p.279-284
Abstract

Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoantibody, Blood coagulation factor, Enzyme-linked immunosorbent assay, Inhibitor
in
Biochemia Medica
volume
26
issue
2
pages
6 pages
publisher
Biochemia Medica, Editorial Office
external identifiers
  • scopus:84973456477
  • wos:000377907400020
ISSN
1330-0962
DOI
10.11613/BM.2016.033
language
English
LU publication?
no
id
e61fc36b-e931-4094-9f44-66b768544295
date added to LUP
2016-07-12 15:02:48
date last changed
2017-01-01 08:30:21
@article{e61fc36b-e931-4094-9f44-66b768544295,
  abstract     = {<p>Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.</p>},
  author       = {Saša, Anžej Doma and Andreas, Hillarp and Tadej, Pajič and Dušan, Andoljšek and Peter, Černelč and Irena, Preložnik Zupan},
  issn         = {1330-0962},
  keyword      = {Autoantibody,Blood coagulation factor,Enzyme-linked immunosorbent assay,Inhibitor},
  language     = {eng},
  number       = {2},
  pages        = {279--284},
  publisher    = {Biochemia Medica, Editorial Office},
  series       = {Biochemia Medica},
  title        = {Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report},
  url          = {http://dx.doi.org/10.11613/BM.2016.033},
  volume       = {26},
  year         = {2016},
}