Prognostic implications of various models for calculation of S-phase fraction in 259 patients with soft tissue sarcoma
(1999) In British Journal of Cancer 79. p.1205-1209- Abstract
- The S-phase fraction (SPF) in flow cytometric DNA histograms in soft tissue sarcoma (STS) can be calculated in various ways. The traditional planimetric method of Baisch has been shown to be prognostic, but is hampered by a failure rate of around 40%. We therefore tested other models to see if this rate could be decreased with retained prognostic value. In 259 STS of the locomotor system the SPF was calculated according to Baisch and with commercial parametric MultiCycle software using different corrections for background. Using the Baisch model, 159 histograms could be evaluated for SPF. The 5-year metastasis-free survival rate (MFSR) was 0.94 for the low-risk group (defined with SPF), and 0.53 for the high-risk group. In the low-risk... (More)
- The S-phase fraction (SPF) in flow cytometric DNA histograms in soft tissue sarcoma (STS) can be calculated in various ways. The traditional planimetric method of Baisch has been shown to be prognostic, but is hampered by a failure rate of around 40%. We therefore tested other models to see if this rate could be decreased with retained prognostic value. In 259 STS of the locomotor system the SPF was calculated according to Baisch and with commercial parametric MultiCycle software using different corrections for background. Using the Baisch model, 159 histograms could be evaluated for SPF. The 5-year metastasis-free survival rate (MFSR) was 0.94 for the low-risk group (defined with SPF), and 0.53 for the high-risk group. In the low-risk group, four of the seven patients who developed metastasis did so after 5 years Using the MultiCycle software, SPF could be calculated in 253 tumours. Depending on type of background correction used, the 5-year MFSR varied between 0.67 and 0.82 for the low-risk group, and between 0.47 and 0.53 for the high-risk group. The late metastasis pattern in the low-risk group was never seen using the MultiCycle software. We conclude that in paraffin archival material, calculation of SPF according to Baisch is preferable in clinical use due to better separation between low-risk and high-risk groups, and also the possibility to identify patients who metastasize late. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114342
- author
- Gustafson, Pelle LU ; Baldetorp, Bo LU ; Fernö, Mårten LU and Åkerman, Måns LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- prognosis, background correction, S-phase fraction, soft tissue sarcoma, DNA flow cytometry
- in
- British Journal of Cancer
- volume
- 79
- pages
- 1205 - 1209
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:10098760
- scopus:0033058013
- pmid:10098760
- ISSN
- 1532-1827
- DOI
- 10.1038/sj.bjc.6690193
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Oncology, MV (013035000), Pathology, (Lund) (013030000), Department of Orthopaedics (Lund) (013028000)
- id
- e66c6981-e411-4808-92d4-b7896418eb1d (old id 1114342)
- date added to LUP
- 2016-04-01 12:33:46
- date last changed
- 2022-01-27 06:49:34
@article{e66c6981-e411-4808-92d4-b7896418eb1d, abstract = {{The S-phase fraction (SPF) in flow cytometric DNA histograms in soft tissue sarcoma (STS) can be calculated in various ways. The traditional planimetric method of Baisch has been shown to be prognostic, but is hampered by a failure rate of around 40%. We therefore tested other models to see if this rate could be decreased with retained prognostic value. In 259 STS of the locomotor system the SPF was calculated according to Baisch and with commercial parametric MultiCycle software using different corrections for background. Using the Baisch model, 159 histograms could be evaluated for SPF. The 5-year metastasis-free survival rate (MFSR) was 0.94 for the low-risk group (defined with SPF), and 0.53 for the high-risk group. In the low-risk group, four of the seven patients who developed metastasis did so after 5 years Using the MultiCycle software, SPF could be calculated in 253 tumours. Depending on type of background correction used, the 5-year MFSR varied between 0.67 and 0.82 for the low-risk group, and between 0.47 and 0.53 for the high-risk group. The late metastasis pattern in the low-risk group was never seen using the MultiCycle software. We conclude that in paraffin archival material, calculation of SPF according to Baisch is preferable in clinical use due to better separation between low-risk and high-risk groups, and also the possibility to identify patients who metastasize late.}}, author = {{Gustafson, Pelle and Baldetorp, Bo and Fernö, Mårten and Åkerman, Måns}}, issn = {{1532-1827}}, keywords = {{prognosis; background correction; S-phase fraction; soft tissue sarcoma; DNA flow cytometry}}, language = {{eng}}, pages = {{1205--1209}}, publisher = {{Nature Publishing Group}}, series = {{British Journal of Cancer}}, title = {{Prognostic implications of various models for calculation of S-phase fraction in 259 patients with soft tissue sarcoma}}, url = {{http://dx.doi.org/10.1038/sj.bjc.6690193}}, doi = {{10.1038/sj.bjc.6690193}}, volume = {{79}}, year = {{1999}}, }