Design of high-density antibody microarrays for disease proteomics: Key technological issues.
(2009) In Journal of Proteomics 72(6). p.928-935- Abstract
- Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody... (More)
- Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1412094
- author
- Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Proteomics
- volume
- 72
- issue
- 6
- pages
- 928 - 935
- publisher
- Elsevier
- external identifiers
-
- wos:000269429800003
- pmid:19457338
- scopus:67651161904
- ISSN
- 1874-3919
- DOI
- 10.1016/j.jprot.2009.01.027
- language
- English
- LU publication?
- yes
- id
- e66eb26c-d0d8-47f8-9829-a39d99ae2ca1 (old id 1412094)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19457338?dopt=Abstract
- date added to LUP
- 2016-04-01 12:27:50
- date last changed
- 2022-01-27 05:26:34
@article{e66eb26c-d0d8-47f8-9829-a39d99ae2ca1, abstract = {{Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays.}}, author = {{Borrebaeck, Carl and Wingren, Christer}}, issn = {{1874-3919}}, language = {{eng}}, number = {{6}}, pages = {{928--935}}, publisher = {{Elsevier}}, series = {{Journal of Proteomics}}, title = {{Design of high-density antibody microarrays for disease proteomics: Key technological issues.}}, url = {{http://dx.doi.org/10.1016/j.jprot.2009.01.027}}, doi = {{10.1016/j.jprot.2009.01.027}}, volume = {{72}}, year = {{2009}}, }