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Uncovering the hidden diversity of litter-decomposition mechanisms in mushroom-forming fungi

Floudas, Dimitrios LU ; Bentzer, Johan LU ; Ahrén, Dag LU ; Johansson, Tomas LU ; Persson, Per LU and Tunlid, Anders LU (2020) In ISME Journal
Abstract

Litter decomposing Agaricales play key role in terrestrial carbon cycling, but little is known about their decomposition mechanisms. We assembled datasets of 42 gene families involved in plant-cell-wall decomposition from seven newly sequenced litter decomposers and 35 other Agaricomycotina members, mostly white-rot and brown-rot species. Using sequence similarity and phylogenetics, we split the families into phylogroups and compared their gene composition across nutritional strategies. Subsequently, we used Raman spectroscopy to examine the ability of litter decomposers, white-rot fungi, and brown-rot fungi to decompose crystalline cellulose. Both litter decomposers and white-rot fungi share the enzymatic cellulose decomposition,... (More)

Litter decomposing Agaricales play key role in terrestrial carbon cycling, but little is known about their decomposition mechanisms. We assembled datasets of 42 gene families involved in plant-cell-wall decomposition from seven newly sequenced litter decomposers and 35 other Agaricomycotina members, mostly white-rot and brown-rot species. Using sequence similarity and phylogenetics, we split the families into phylogroups and compared their gene composition across nutritional strategies. Subsequently, we used Raman spectroscopy to examine the ability of litter decomposers, white-rot fungi, and brown-rot fungi to decompose crystalline cellulose. Both litter decomposers and white-rot fungi share the enzymatic cellulose decomposition, whereas brown-rot fungi possess a distinct mechanism that disrupts cellulose crystallinity. However, litter decomposers and white-rot fungi differ with respect to hemicellulose and lignin degradation phylogroups, suggesting adaptation of the former group to the litter environment. Litter decomposers show high phylogroup diversity, which is indicative of high functional versatility within the group, whereas a set of white-rot species shows adaptation to bulk-wood decomposition. In both groups, we detected species that have unique characteristics associated with hitherto unknown adaptations to diverse wood and litter substrates. Our results suggest that the terms white-rot fungi and litter decomposers mask a much larger functional diversity.

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Contribution to journal
publication status
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in
ISME Journal
publisher
Nature Publishing Group
external identifiers
  • pmid:32382073
  • scopus:85085078624
ISSN
1751-7362
DOI
10.1038/s41396-020-0667-6
language
English
LU publication?
yes
id
e705db55-be09-4a25-b818-5dafc506f082
date added to LUP
2020-06-25 15:00:48
date last changed
2020-06-26 03:00:04
@article{e705db55-be09-4a25-b818-5dafc506f082,
  abstract     = {<p>Litter decomposing Agaricales play key role in terrestrial carbon cycling, but little is known about their decomposition mechanisms. We assembled datasets of 42 gene families involved in plant-cell-wall decomposition from seven newly sequenced litter decomposers and 35 other Agaricomycotina members, mostly white-rot and brown-rot species. Using sequence similarity and phylogenetics, we split the families into phylogroups and compared their gene composition across nutritional strategies. Subsequently, we used Raman spectroscopy to examine the ability of litter decomposers, white-rot fungi, and brown-rot fungi to decompose crystalline cellulose. Both litter decomposers and white-rot fungi share the enzymatic cellulose decomposition, whereas brown-rot fungi possess a distinct mechanism that disrupts cellulose crystallinity. However, litter decomposers and white-rot fungi differ with respect to hemicellulose and lignin degradation phylogroups, suggesting adaptation of the former group to the litter environment. Litter decomposers show high phylogroup diversity, which is indicative of high functional versatility within the group, whereas a set of white-rot species shows adaptation to bulk-wood decomposition. In both groups, we detected species that have unique characteristics associated with hitherto unknown adaptations to diverse wood and litter substrates. Our results suggest that the terms white-rot fungi and litter decomposers mask a much larger functional diversity.</p>},
  author       = {Floudas, Dimitrios and Bentzer, Johan and Ahrén, Dag and Johansson, Tomas and Persson, Per and Tunlid, Anders},
  issn         = {1751-7362},
  language     = {eng},
  month        = {05},
  publisher    = {Nature Publishing Group},
  series       = {ISME Journal},
  title        = {Uncovering the hidden diversity of litter-decomposition mechanisms in mushroom-forming fungi},
  url          = {http://dx.doi.org/10.1038/s41396-020-0667-6},
  doi          = {10.1038/s41396-020-0667-6},
  year         = {2020},
}