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Single-cell sequencing in translational cancer research and challenges to meet clinical diagnostic needs

Pfisterer, Ulrich LU ; Bräunig, Julia LU ; Brattås, Per LU ; Heidenblad, Markus LU ; Karlsson, Göran LU and Fioretos, Thoas LU (2021) In Genes, Chromosomes and Cancer 60(7). p.504-524
Abstract

The ability to capture alterations in the genome or transcriptome by next-generation sequencing has provided critical insight into molecular changes and programs underlying cancer biology. With the rapid technological development in single-cell sequencing, it has become possible to study individual cells at the transcriptional, genetic, epigenetic, and protein level. Using single-cell analysis, an increased resolution of fundamental processes underlying cancer development is obtained, providing comprehensive insights otherwise lost by sequencing of entire (bulk) samples, in which molecular signatures of individual cells are averaged across the entire cell population. Here, we provide a concise overview on the application of single-cell... (More)

The ability to capture alterations in the genome or transcriptome by next-generation sequencing has provided critical insight into molecular changes and programs underlying cancer biology. With the rapid technological development in single-cell sequencing, it has become possible to study individual cells at the transcriptional, genetic, epigenetic, and protein level. Using single-cell analysis, an increased resolution of fundamental processes underlying cancer development is obtained, providing comprehensive insights otherwise lost by sequencing of entire (bulk) samples, in which molecular signatures of individual cells are averaged across the entire cell population. Here, we provide a concise overview on the application of single-cell analysis of different modalities within cancer research by highlighting key articles of their respective fields. We furthermore examine the potential of existing technologies to meet clinical diagnostic needs and discuss current challenges associated with this translation.

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Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Genes, Chromosomes and Cancer
volume
60
issue
7
pages
21 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:33611828
  • scopus:85102467396
ISSN
1045-2257
DOI
10.1002/gcc.22944
language
English
LU publication?
yes
id
e7315982-eb86-4e80-9d3a-4dfb515e02ed
date added to LUP
2021-03-26 12:12:44
date last changed
2024-12-29 05:14:25
@article{e7315982-eb86-4e80-9d3a-4dfb515e02ed,
  abstract     = {{<p>The ability to capture alterations in the genome or transcriptome by next-generation sequencing has provided critical insight into molecular changes and programs underlying cancer biology. With the rapid technological development in single-cell sequencing, it has become possible to study individual cells at the transcriptional, genetic, epigenetic, and protein level. Using single-cell analysis, an increased resolution of fundamental processes underlying cancer development is obtained, providing comprehensive insights otherwise lost by sequencing of entire (bulk) samples, in which molecular signatures of individual cells are averaged across the entire cell population. Here, we provide a concise overview on the application of single-cell analysis of different modalities within cancer research by highlighting key articles of their respective fields. We furthermore examine the potential of existing technologies to meet clinical diagnostic needs and discuss current challenges associated with this translation.</p>}},
  author       = {{Pfisterer, Ulrich and Bräunig, Julia and Brattås, Per and Heidenblad, Markus and Karlsson, Göran and Fioretos, Thoas}},
  issn         = {{1045-2257}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{7}},
  pages        = {{504--524}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Genes, Chromosomes and Cancer}},
  title        = {{Single-cell sequencing in translational cancer research and challenges to meet clinical diagnostic needs}},
  url          = {{http://dx.doi.org/10.1002/gcc.22944}},
  doi          = {{10.1002/gcc.22944}},
  volume       = {{60}},
  year         = {{2021}},
}