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Effect of impaired glucose tolerance on atherosclerotic lesion formation : An evaluation in selectively bred mice with different susceptibilities to glucose intolerance

Asai, Akira; Nagao, Mototsugu LU ; Kawahara, Momoyo; Shuto, Yuki; Sugihara, Hitoshi and Oikawa, Shinichi (2013) In Atherosclerosis 231(2). p.421-426
Abstract

Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28... (More)

Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28 weeks of age). Oral glucose tolerance tests were performed during the AD-feeding period. Atherosclerotic lesion formation was quantitatively analyzed in serial aortic sinus sections by oil red O staining. Plasma lipids were measured after the AD-feeding period. Results: Glucose tolerance was impaired in SDG-P mice as compared to SDG-R mice over the 20-week AD-feeding period. No significant differences were observed in any plasma lipid measurement between the 2 mouse lines. Aortic sinus atherosclerotic lesion formation in SDG-P mice was approximately 4-fold greater than that in SDG-R mice. Conclusion: In 2 mouse lines with different susceptibilities to diet-induced glucose intolerance, IGT accelerated atherosclerotic lesion formation. These mice may therefore serve as useful invivo models for investigating the causal role of IGT in the pathogenesis of atherosclerosis.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal model, Atherosclerosis, Impaired glucose tolerance, Postprandial hyperglycemia
in
Atherosclerosis
volume
231
issue
2
pages
421 - 426
publisher
Elsevier
external identifiers
  • scopus:84888146994
ISSN
0021-9150
DOI
10.1016/j.atherosclerosis.2013.10.009
language
English
LU publication?
no
id
e736c470-39b6-4619-90e5-23a27c2979a8
date added to LUP
2017-08-23 20:03:06
date last changed
2018-05-29 09:36:44
@article{e736c470-39b6-4619-90e5-23a27c2979a8,
  abstract     = {<p>Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28 weeks of age). Oral glucose tolerance tests were performed during the AD-feeding period. Atherosclerotic lesion formation was quantitatively analyzed in serial aortic sinus sections by oil red O staining. Plasma lipids were measured after the AD-feeding period. Results: Glucose tolerance was impaired in SDG-P mice as compared to SDG-R mice over the 20-week AD-feeding period. No significant differences were observed in any plasma lipid measurement between the 2 mouse lines. Aortic sinus atherosclerotic lesion formation in SDG-P mice was approximately 4-fold greater than that in SDG-R mice. Conclusion: In 2 mouse lines with different susceptibilities to diet-induced glucose intolerance, IGT accelerated atherosclerotic lesion formation. These mice may therefore serve as useful invivo models for investigating the causal role of IGT in the pathogenesis of atherosclerosis.</p>},
  author       = {Asai, Akira and Nagao, Mototsugu and Kawahara, Momoyo and Shuto, Yuki and Sugihara, Hitoshi and Oikawa, Shinichi},
  issn         = {0021-9150},
  keyword      = {Animal model,Atherosclerosis,Impaired glucose tolerance,Postprandial hyperglycemia},
  language     = {eng},
  number       = {2},
  pages        = {421--426},
  publisher    = {Elsevier},
  series       = {Atherosclerosis},
  title        = {Effect of impaired glucose tolerance on atherosclerotic lesion formation : An evaluation in selectively bred mice with different susceptibilities to glucose intolerance},
  url          = {http://dx.doi.org/10.1016/j.atherosclerosis.2013.10.009},
  volume       = {231},
  year         = {2013},
}