Plaque-associated lipids in Alzheimer's diseased brain tissue visualized by nonlinear microscopy.
(2015) In Scientific Reports 5.- Abstract
- By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with... (More)
- By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with the β-sheet vibrations. The lipid fluidity was evaluated from the CARS spectra, illustrating that the lipid composition/organization varies throughout the plaques. Altogether this indicates close amyloid-lipid interplay in fibrillar Aβ plaques, rendering them more dynamic compositions than previously believed and, hence, potential sources of toxic oligomers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7834335
- author
- Kiskis, Juris ; Fink, Helen ; Nyberg, Lena ; Thyr, Jacob ; Li, Jia-Yi LU and Enejder, Annika
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 5
- article number
- 13489
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000360144800001
- pmid:26311128
- scopus:84940510248
- pmid:26311128
- ISSN
- 2045-2322
- DOI
- 10.1038/srep13489
- language
- English
- LU publication?
- yes
- id
- e75a5a09-f7eb-421f-8e24-1af2fff6d6a2 (old id 7834335)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26311128?dopt=Abstract
- date added to LUP
- 2016-04-01 14:44:44
- date last changed
- 2022-05-15 20:23:40
@article{e75a5a09-f7eb-421f-8e24-1af2fff6d6a2,
abstract = {{By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with the β-sheet vibrations. The lipid fluidity was evaluated from the CARS spectra, illustrating that the lipid composition/organization varies throughout the plaques. Altogether this indicates close amyloid-lipid interplay in fibrillar Aβ plaques, rendering them more dynamic compositions than previously believed and, hence, potential sources of toxic oligomers.}},
author = {{Kiskis, Juris and Fink, Helen and Nyberg, Lena and Thyr, Jacob and Li, Jia-Yi and Enejder, Annika}},
issn = {{2045-2322}},
language = {{eng}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Plaque-associated lipids in Alzheimer's diseased brain tissue visualized by nonlinear microscopy.}},
url = {{https://lup.lub.lu.se/search/files/4140210/8626773.pdf}},
doi = {{10.1038/srep13489}},
volume = {{5}},
year = {{2015}},
}