Advanced

Chemical and Structural Changes in Corn Stover After Ensiling : Influence on Bioconversion

Nagle, Nick J. ; Donohoe, Bryon S. ; Wolfrum, Edward J. ; Kuhn, Erik M. ; Haas, Thomas J. ; Ray, Allison E. ; Wendt, Lynn M. ; Delwiche, Mark E. ; Weiss, Noah D. LU and Radtke, Corey (2020) In Frontiers in Bioengineering and Biotechnology 8.
Abstract

Production of biofuels, bioproducts, and bioenergy requires a well-characterized, stable, and reasonably uniform biomass supply and well-established supply chains for shipping biomass from farm fields to biorefineries, while achieving year-round production targets. Preserving and stabilizing biomass feedstock during storage is a necessity for cost-effective and sustainable biofuel production. Ensiling is a common storage method used to preserve and even improve forage quality; however, the impact of ensiling on biomass physical and chemical properties that influence bioconversion processes has been variable. Our objective in this work was to determine the effects of ensiling on lignocellulosic feedstock physicochemical properties and... (More)

Production of biofuels, bioproducts, and bioenergy requires a well-characterized, stable, and reasonably uniform biomass supply and well-established supply chains for shipping biomass from farm fields to biorefineries, while achieving year-round production targets. Preserving and stabilizing biomass feedstock during storage is a necessity for cost-effective and sustainable biofuel production. Ensiling is a common storage method used to preserve and even improve forage quality; however, the impact of ensiling on biomass physical and chemical properties that influence bioconversion processes has been variable. Our objective in this work was to determine the effects of ensiling on lignocellulosic feedstock physicochemical properties and how that influences bioconversion requirements. We observed statistically significant decreases (p < 0.05) in the content of two major structural carbohydrates (glucan and xylan) of 5 and 8%, respectively, between the ensiled and non-ensiled materials. We were unable to detect differences in sugar yields from structural carbohydrates after pretreatment and enzymatic hydrolysis of the ensiled materials compared to non-ensiled controls. Based on this work, we conclude that ensiling the corn stover did not change the bioconversion requirements compared to the control samples and incurred losses of structural carbohydrates. At the light microscopy level, ensiled corn stover exhibited little structural change or relocation of cell wall components as detected by immunocytochemistry. However, more subtle structural changes were revealed by electron microscopy, as ensiled cell walls exhibit ultrastructural characteristics such as wall delimitation intermediate between non-ensiled and dilute-acid-pretreated cell walls. These findings suggest that alternative methods of conversion, such as deacetylation and mechanical refining, could take advantage of lamellar defects and may be more effective than dilute acid or hot water pretreatment for biomass conversion of ensiled materials.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioconversion, bioproducts, ensiling, ethanol, feedstock logistics, pretreatment
in
Frontiers in Bioengineering and Biotechnology
volume
8
article number
739
publisher
Frontiers Media S. A.
external identifiers
  • pmid:32923429
  • scopus:85090005489
ISSN
2296-4185
DOI
10.3389/fbioe.2020.00739
language
English
LU publication?
yes
id
e77bf6c1-16cb-4985-bba5-3365eb630364
date added to LUP
2020-09-25 15:26:27
date last changed
2021-02-23 01:44:16
@article{e77bf6c1-16cb-4985-bba5-3365eb630364,
  abstract     = {<p>Production of biofuels, bioproducts, and bioenergy requires a well-characterized, stable, and reasonably uniform biomass supply and well-established supply chains for shipping biomass from farm fields to biorefineries, while achieving year-round production targets. Preserving and stabilizing biomass feedstock during storage is a necessity for cost-effective and sustainable biofuel production. Ensiling is a common storage method used to preserve and even improve forage quality; however, the impact of ensiling on biomass physical and chemical properties that influence bioconversion processes has been variable. Our objective in this work was to determine the effects of ensiling on lignocellulosic feedstock physicochemical properties and how that influences bioconversion requirements. We observed statistically significant decreases (p &lt; 0.05) in the content of two major structural carbohydrates (glucan and xylan) of 5 and 8%, respectively, between the ensiled and non-ensiled materials. We were unable to detect differences in sugar yields from structural carbohydrates after pretreatment and enzymatic hydrolysis of the ensiled materials compared to non-ensiled controls. Based on this work, we conclude that ensiling the corn stover did not change the bioconversion requirements compared to the control samples and incurred losses of structural carbohydrates. At the light microscopy level, ensiled corn stover exhibited little structural change or relocation of cell wall components as detected by immunocytochemistry. However, more subtle structural changes were revealed by electron microscopy, as ensiled cell walls exhibit ultrastructural characteristics such as wall delimitation intermediate between non-ensiled and dilute-acid-pretreated cell walls. These findings suggest that alternative methods of conversion, such as deacetylation and mechanical refining, could take advantage of lamellar defects and may be more effective than dilute acid or hot water pretreatment for biomass conversion of ensiled materials.</p>},
  author       = {Nagle, Nick J. and Donohoe, Bryon S. and Wolfrum, Edward J. and Kuhn, Erik M. and Haas, Thomas J. and Ray, Allison E. and Wendt, Lynn M. and Delwiche, Mark E. and Weiss, Noah D. and Radtke, Corey},
  issn         = {2296-4185},
  language     = {eng},
  publisher    = {Frontiers Media S. A.},
  series       = {Frontiers in Bioengineering and Biotechnology},
  title        = {Chemical and Structural Changes in Corn Stover After Ensiling : Influence on Bioconversion},
  url          = {http://dx.doi.org/10.3389/fbioe.2020.00739},
  doi          = {10.3389/fbioe.2020.00739},
  volume       = {8},
  year         = {2020},
}