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High-throughput analysis of the structural evolution of the monoolein cubic phase in situ under crystallogenesis conditions

Conn, Charlotte E.; Darmanin, Connie; Mulet, Xavier; Le Cann, Sophie LU ; Kirby, Nigel and Drummond, Calum J. (2012) In Soft Matter 8(7). p.2310-2321
Abstract
We have tracked the structural evolution of the monoolein bicontinuous cubic phase under in meso crystallogenesis conditions. Significantly, all measurements have been carried out in situ within a crystallisation plate reproducing the exact conditions during a crystallisation trial. The structure of the MO cubic phase, doped with a small concentration of amyloid-beta peptide, was measured 1 day, 5 days, 7 days and 21 days after addition of PACT crystallisation screen, which systematically varies cation, anion, pH and polyethylene glycol (PEG). The components of the screen had a significant impact on the structure of the cubic phase. We have rationalised the structural variation with respect to the effect of the individual screen... (More)
We have tracked the structural evolution of the monoolein bicontinuous cubic phase under in meso crystallogenesis conditions. Significantly, all measurements have been carried out in situ within a crystallisation plate reproducing the exact conditions during a crystallisation trial. The structure of the MO cubic phase, doped with a small concentration of amyloid-beta peptide, was measured 1 day, 5 days, 7 days and 21 days after addition of PACT crystallisation screen, which systematically varies cation, anion, pH and polyethylene glycol (PEG). The components of the screen had a significant impact on the structure of the cubic phase. We have rationalised the structural variation with respect to the effect of the individual screen components. Specifically addition of higher Mw PEG effected a transition from a diamond cubic phase (QIID) to a gyroid cubic phase (QIIG) across the majority of the plate and the QIIG phase became more prevalent with time. The effect of individual salts was correlated with their position in the Hofmeister series. Changes in pH and buffer system had a more minor effect on mesophase structure. We have discussed the implications of the observed structural changes with respect to the putative mechanism of protein crystal growth within a lipidic cubic phase. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
8
issue
7
pages
2310 - 2321
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84856733257
ISSN
1744-6848
DOI
10.1039/C2SM07232G
language
English
LU publication?
no
id
e7d100e5-422f-4adb-b0a6-e7c9ec690eb8
date added to LUP
2017-02-15 11:06:42
date last changed
2017-11-12 04:29:22
@article{e7d100e5-422f-4adb-b0a6-e7c9ec690eb8,
  abstract     = {We have tracked the structural evolution of the monoolein bicontinuous cubic phase under in meso crystallogenesis conditions. Significantly, all measurements have been carried out in situ within a crystallisation plate reproducing the exact conditions during a crystallisation trial. The structure of the MO cubic phase, doped with a small concentration of amyloid-beta peptide, was measured 1 day, 5 days, 7 days and 21 days after addition of PACT crystallisation screen, which systematically varies cation, anion, pH and polyethylene glycol (PEG). The components of the screen had a significant impact on the structure of the cubic phase. We have rationalised the structural variation with respect to the effect of the individual screen components. Specifically addition of higher Mw PEG effected a transition from a diamond cubic phase (QIID) to a gyroid cubic phase (QIIG) across the majority of the plate and the QIIG phase became more prevalent with time. The effect of individual salts was correlated with their position in the Hofmeister series. Changes in pH and buffer system had a more minor effect on mesophase structure. We have discussed the implications of the observed structural changes with respect to the putative mechanism of protein crystal growth within a lipidic cubic phase.},
  author       = {Conn, Charlotte E. and Darmanin, Connie and Mulet, Xavier and Le Cann, Sophie and Kirby, Nigel and Drummond, Calum J.},
  issn         = {1744-6848},
  language     = {eng},
  number       = {7},
  pages        = {2310--2321},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {High-throughput analysis of the structural evolution of the monoolein cubic phase in situ under crystallogenesis conditions},
  url          = {http://dx.doi.org/10.1039/C2SM07232G},
  volume       = {8},
  year         = {2012},
}