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Synthesis of the bifuncttonal dinucleotide AMP-ATP and its application in general ligand affinity chromatography

Lee, Chi-yu ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1977) In Journal of Solid-Phase Biochemistry 2(1). p.31-39
Abstract
A new AMP derivative substituted with spacer arms both at position N6 and C8 of the adenine moiety was synthesized and immobilized to Sepharose. To the immobilized ligand was subsequently coupled C8-substituted ATP in a solid-phase synthesis fashion yielding the bifunctional general ligand AMP-ATP. This affinity material was used in the separation of two major groups of enzymes, dehydrogenases and kinases. It was found that on passage of crude homogenates obtained from mouse kidney through the affinity column, several dehydrogenases and kinases were bound, which could be eluted separately using pulses of NADH and ATP, respectively. In the fractions obtained on NADH elution, lactate dehydrogenase, malate dehydrogenase, and... (More)
A new AMP derivative substituted with spacer arms both at position N6 and C8 of the adenine moiety was synthesized and immobilized to Sepharose. To the immobilized ligand was subsequently coupled C8-substituted ATP in a solid-phase synthesis fashion yielding the bifunctional general ligand AMP-ATP. This affinity material was used in the separation of two major groups of enzymes, dehydrogenases and kinases. It was found that on passage of crude homogenates obtained from mouse kidney through the affinity column, several dehydrogenases and kinases were bound, which could be eluted separately using pulses of NADH and ATP, respectively. In the fractions obtained on NADH elution, lactate dehydrogenase, malate dehydrogenase, and α-glycerol phosphate dehydrogenase were found, whereas ATP eluted 3-phosphoglyceric acid kinase, pyruvate kinase, and aldolase. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Solid-Phase Biochemistry
volume
2
issue
1
pages
9 pages
publisher
Springer
external identifiers
  • scopus:0017567741
DOI
10.1007/BF02991028
language
English
LU publication?
yes
id
e7e40045-b7c5-42d5-9093-596b792c06e0
date added to LUP
2024-06-22 16:14:40
date last changed
2024-09-13 11:32:00
@article{e7e40045-b7c5-42d5-9093-596b792c06e0,
  abstract     = {{A new AMP derivative substituted with spacer arms both at position N<sup>6</sup> and C8 of the adenine moiety was synthesized and immobilized to Sepharose. To the immobilized ligand was subsequently coupled C8-substituted ATP in a solid-phase synthesis fashion yielding the bifunctional general ligand AMP-ATP. This affinity material was used in the separation of two major groups of enzymes, dehydrogenases and kinases. It was found that on passage of crude homogenates obtained from mouse kidney through the affinity column, several dehydrogenases and kinases were bound, which could be eluted separately using pulses of NADH and ATP, respectively. In the fractions obtained on NADH elution, lactate dehydrogenase, malate dehydrogenase, and α-glycerol phosphate dehydrogenase were found, whereas ATP eluted 3-phosphoglyceric acid kinase, pyruvate kinase, and aldolase.}},
  author       = {{Lee, Chi-yu and Larsson, Per-Olof and Mosbach, Klaus}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{31--39}},
  publisher    = {{Springer}},
  series       = {{Journal of Solid-Phase Biochemistry}},
  title        = {{Synthesis of the bifuncttonal dinucleotide AMP-ATP and its application in general ligand affinity chromatography}},
  url          = {{http://dx.doi.org/10.1007/BF02991028}},
  doi          = {{10.1007/BF02991028}},
  volume       = {{2}},
  year         = {{1977}},
}