BtuB-dependent infection of the T5-like Yersinia phage φr2-01
(2021) In Viruses 13(11). p.1-18- Abstract
Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_φR2-01 (in short, φR2-01) that infects strains of several Yersinia enterocolitica serotypes. The φR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The φR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding... (More)
Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_φR2-01 (in short, φR2-01) that infects strains of several Yersinia enterocolitica serotypes. The φR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The φR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical φR2-01 proteins. Label-free massspectrometry-based proteomics confirmed the expression of 90 of the φR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and φR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of φR2-01 as a food biocontrol or phage therapy agent.
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- author
- Happonen, Lotta J. LU ; Pajunen, Maria I. ; Jun, Jin Woo and Skurnik, Mikael
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacteriophage, BtuB, Escherichia phage T5, Genome, Phage φR2-01, Proteome, Yersinia enterocolitica
- in
- Viruses
- volume
- 13
- issue
- 11
- article number
- 2171
- pages
- 1 - 18
- publisher
- MDPI AG
- external identifiers
-
- scopus:85118409877
- pmid:34834977
- ISSN
- 1999-4915
- DOI
- 10.3390/v13112171
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: This research was funded by the Academy of Finland, grant number 288701, to M.S. Open access funding provided by University of Helsinki. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- e80678e3-84ad-443d-a982-2270e7a49c50
- date added to LUP
- 2021-11-13 09:00:23
- date last changed
- 2024-06-15 20:18:07
@article{e80678e3-84ad-443d-a982-2270e7a49c50,
abstract = {{<p>Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_φR2-01 (in short, φR2-01) that infects strains of several Yersinia enterocolitica serotypes. The φR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The φR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical φR2-01 proteins. Label-free massspectrometry-based proteomics confirmed the expression of 90 of the φR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and φR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of φR2-01 as a food biocontrol or phage therapy agent.</p>}},
author = {{Happonen, Lotta J. and Pajunen, Maria I. and Jun, Jin Woo and Skurnik, Mikael}},
issn = {{1999-4915}},
keywords = {{Bacteriophage; BtuB; Escherichia phage T5; Genome; Phage φR2-01; Proteome; Yersinia enterocolitica}},
language = {{eng}},
number = {{11}},
pages = {{1--18}},
publisher = {{MDPI AG}},
series = {{Viruses}},
title = {{BtuB-dependent infection of the T5-like Yersinia phage φr2-01}},
url = {{http://dx.doi.org/10.3390/v13112171}},
doi = {{10.3390/v13112171}},
volume = {{13}},
year = {{2021}},
}