Quantitative phase measurements of human cell nuclei using X-ray ptychography
(2021) In Journal of Synchrotron Radiation 28. p.1166-1173- Abstract
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also... (More)
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
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- author
- Schwenke, Jorg LU ; Yusuf, Mohammed ; Shemilt, Laura A. ; Wagner, Ulrich ; Sajid, Atiqa ; Morrison, Graeme R. ; Zhang, Fucai ; Parsons, Aaron ; Rau, Christoph and Robinson, Ian K.
- organization
- publishing date
- 2021-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nuclei, Ptychography, Quantitative phase imaging, Synchrotron, X-ray
- in
- Journal of Synchrotron Radiation
- volume
- 28
- pages
- 8 pages
- publisher
- International Union of Crystallography
- external identifiers
-
- scopus:85110320532
- pmid:34212880
- ISSN
- 0909-0495
- DOI
- 10.1107/S1600577521004586
- language
- English
- LU publication?
- yes
- id
- e82b5f6f-36dd-405c-b227-1f59586c89cd
- date added to LUP
- 2022-02-22 16:35:43
- date last changed
- 2024-04-18 05:38:42
@article{e82b5f6f-36dd-405c-b227-1f59586c89cd,
abstract = {{<p>The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.</p>}},
author = {{Schwenke, Jorg and Yusuf, Mohammed and Shemilt, Laura A. and Wagner, Ulrich and Sajid, Atiqa and Morrison, Graeme R. and Zhang, Fucai and Parsons, Aaron and Rau, Christoph and Robinson, Ian K.}},
issn = {{0909-0495}},
keywords = {{Nuclei; Ptychography; Quantitative phase imaging; Synchrotron; X-ray}},
language = {{eng}},
month = {{07}},
pages = {{1166--1173}},
publisher = {{International Union of Crystallography}},
series = {{Journal of Synchrotron Radiation}},
title = {{Quantitative phase measurements of human cell nuclei using X-ray ptychography}},
url = {{http://dx.doi.org/10.1107/S1600577521004586}},
doi = {{10.1107/S1600577521004586}},
volume = {{28}},
year = {{2021}},
}