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Intracellular K+ Determination With a Potentiometric Microelectrode Based on ZnO Nanowires

Ali, Syed Muhammad Usman; Asif, Muhammad H.; Fulati, Alimujiang; Nur, Omer; Willander, Magnus; Brannmark, Cecilia; Stralfors, Peter; Englund, Ulrika H.; Elinder, Fredrik and Danielsson, Bengt LU (2011) In IEEE Transactions on Nanotechnology 10(4). p.913-919
Abstract
The fabrication and application of an intracellular K+-selective microelectrode is demonstrated. ZnO nanowires with a diameter of 100-180 nm and a length of approximately 1.5. m are grown on a borosilicate glass microcapillary. The ZnO nanowires were coated by a K+-ionophore-containing membrane. The K+-selective microelectrode exhibited a K+-dependent potentiometric response versus an Ag/AgCl reference microelectrode that was linear over a large concentration range (25 . M-125 mM) with a minimum detection limit of 1 . M. The measured K+ concentrations in human adipocytes and in frog oocytes were consistent with values of K+ concentrations reported in the literature. The sensor has several advantages including ease of fabrication, ease of... (More)
The fabrication and application of an intracellular K+-selective microelectrode is demonstrated. ZnO nanowires with a diameter of 100-180 nm and a length of approximately 1.5. m are grown on a borosilicate glass microcapillary. The ZnO nanowires were coated by a K+-ionophore-containing membrane. The K+-selective microelectrode exhibited a K+-dependent potentiometric response versus an Ag/AgCl reference microelectrode that was linear over a large concentration range (25 . M-125 mM) with a minimum detection limit of 1 . M. The measured K+ concentrations in human adipocytes and in frog oocytes were consistent with values of K+ concentrations reported in the literature. The sensor has several advantages including ease of fabrication, ease of insertion into the cells, low cost, and high selectivity features that make this type of sensor suitable to characterize physiologically relevant ions within single living cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Frog oocytes, human adipocytes, intracellular potassium ions, ionophore, membrane, nanotechnology, potentiometric nanosensor, ZnO nanowires
in
IEEE Transactions on Nanotechnology
volume
10
issue
4
pages
913 - 919
publisher
IEEE--Institute of Electrical and Electronics Engineers Inc.
external identifiers
  • wos:000292966400038
  • scopus:79960258617
ISSN
1536-125X
DOI
10.1109/TNANO.2010.2089696
language
English
LU publication?
yes
id
e850d521-e7cf-4579-8b14-561f964109bd (old id 2091613)
date added to LUP
2011-08-25 14:10:40
date last changed
2017-12-10 04:23:57
@article{e850d521-e7cf-4579-8b14-561f964109bd,
  abstract     = {The fabrication and application of an intracellular K+-selective microelectrode is demonstrated. ZnO nanowires with a diameter of 100-180 nm and a length of approximately 1.5. m are grown on a borosilicate glass microcapillary. The ZnO nanowires were coated by a K+-ionophore-containing membrane. The K+-selective microelectrode exhibited a K+-dependent potentiometric response versus an Ag/AgCl reference microelectrode that was linear over a large concentration range (25 . M-125 mM) with a minimum detection limit of 1 . M. The measured K+ concentrations in human adipocytes and in frog oocytes were consistent with values of K+ concentrations reported in the literature. The sensor has several advantages including ease of fabrication, ease of insertion into the cells, low cost, and high selectivity features that make this type of sensor suitable to characterize physiologically relevant ions within single living cells.},
  author       = {Ali, Syed Muhammad Usman and Asif, Muhammad H. and Fulati, Alimujiang and Nur, Omer and Willander, Magnus and Brannmark, Cecilia and Stralfors, Peter and Englund, Ulrika H. and Elinder, Fredrik and Danielsson, Bengt},
  issn         = {1536-125X},
  keyword      = {Frog oocytes,human adipocytes,intracellular potassium ions,ionophore,membrane,nanotechnology,potentiometric nanosensor,ZnO nanowires},
  language     = {eng},
  number       = {4},
  pages        = {913--919},
  publisher    = {IEEE--Institute of Electrical and Electronics Engineers Inc.},
  series       = {IEEE Transactions on Nanotechnology},
  title        = {Intracellular K+ Determination With a Potentiometric Microelectrode Based on ZnO Nanowires},
  url          = {http://dx.doi.org/10.1109/TNANO.2010.2089696},
  volume       = {10},
  year         = {2011},
}