Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay
(2015) In Biotechnology Journal 10(3). p.460-468- Abstract
- Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 x 10(4) bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 W. The growth rate... (More)
- Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 x 10(4) bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 W. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h(-1), respectively. Additional insight on protease activities of P. mirabilis matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 W was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 m. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5293696
- author
- Braissant, Olivier ; Keiser, Jennifer ; Meister, Isabel ; Bachmann, Alexander ; Wirz, Dieter ; Goepfert, Beat ; Bonkat, Gernot and Wadsö, Ingemar LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Analytical biotechnology, Cell biology, Diseases, Isothermal, microcalorimetry, Metabolic flux analysis
- in
- Biotechnology Journal
- volume
- 10
- issue
- 3
- pages
- 460 - 468
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000351366600012
- scopus:84924606514
- pmid:25511812
- ISSN
- 1860-6768
- DOI
- 10.1002/biot.201400494
- language
- English
- LU publication?
- yes
- id
- e878eb9b-cbca-44dd-8774-28852df16e20 (old id 5293696)
- date added to LUP
- 2016-04-01 10:34:38
- date last changed
- 2022-04-04 19:26:20
@article{e878eb9b-cbca-44dd-8774-28852df16e20,
abstract = {{Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 x 10(4) bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 W. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h(-1), respectively. Additional insight on protease activities of P. mirabilis matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 W was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 m. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm.}},
author = {{Braissant, Olivier and Keiser, Jennifer and Meister, Isabel and Bachmann, Alexander and Wirz, Dieter and Goepfert, Beat and Bonkat, Gernot and Wadsö, Ingemar}},
issn = {{1860-6768}},
keywords = {{Analytical biotechnology; Cell biology; Diseases; Isothermal; microcalorimetry; Metabolic flux analysis}},
language = {{eng}},
number = {{3}},
pages = {{460--468}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology Journal}},
title = {{Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay}},
url = {{http://dx.doi.org/10.1002/biot.201400494}},
doi = {{10.1002/biot.201400494}},
volume = {{10}},
year = {{2015}},
}