Preparation of protein imprinted polymer beads by Pickering emulsion polymerization
Zhou, Tongchang LU ; Zhang, Ka LU ; Kamra, Tripta LU ; Bülow, Leif LU and Ye, Lei LU
(2015)
In Journal of Materials Chemistry. B
3(7).
p.1254-1260
- Abstract
- We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion... (More)
- We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5168988
- author
- Zhou, Tongchang
LU
; Zhang, Ka
LU
; Kamra, Tripta
LU
; Bülow, Leif
LU
and Ye, Lei
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Materials Chemistry. B
- volume
- 3
- issue
- 7
- pages
- 1254 - 1260
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000349617600010
- scopus:84929459850
- ISSN
- 2050-7518
- DOI
- 10.1039/c4tb01605j
- language
- English
- LU publication?
- yes
- id
- e913862b-0dc4-4d2e-9ffe-87da22128421 (old id 5168988)
- date added to LUP
- 2016-04-01 10:49:14
- date last changed
- 2022-02-17 21:39:28
@article{e913862b-0dc4-4d2e-9ffe-87da22128421,
abstract = {{We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.}},
author = {{Zhou, Tongchang and Zhang, Ka and Kamra, Tripta and Bülow, Leif and Ye, Lei}},
issn = {{2050-7518}},
language = {{eng}},
number = {{7}},
pages = {{1254--1260}},
publisher = {{Royal Society of Chemistry}},
series = {{Journal of Materials Chemistry. B}},
title = {{Preparation of protein imprinted polymer beads by Pickering emulsion polymerization}},
url = {{http://dx.doi.org/10.1039/c4tb01605j}},
doi = {{10.1039/c4tb01605j}},
volume = {{3}},
year = {{2015}},
}