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Specific monitoring of chromatographic procedures

Danielsson, B. LU and Larsson, P.-O. LU (1990) In TrAC - Trends in Analytical Chemistry 9(7). p.223-227
Abstract
On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor instrument as the detector of enzymic activity, has definite advantages. Moreover, the same instrument can be utilized in an automated, rapid on-line thermometric immunoassay for biomolecules other than enzymes.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
TrAC - Trends in Analytical Chemistry
volume
9
issue
7
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:0025476988
ISSN
0165-9936
DOI
10.1016/0165-9936(90)87125-6
language
English
LU publication?
yes
id
e928c3d8-02ae-44af-8fec-baf862d5f2c0
date added to LUP
2024-06-17 13:54:06
date last changed
2024-08-29 12:06:01
@article{e928c3d8-02ae-44af-8fec-baf862d5f2c0,
  abstract     = {{On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor instrument as the detector of enzymic activity, has definite advantages. Moreover, the same instrument can be utilized in an automated, rapid on-line thermometric immunoassay for biomolecules other than enzymes.}},
  author       = {{Danielsson, B. and Larsson, P.-O.}},
  issn         = {{0165-9936}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{223--227}},
  publisher    = {{Elsevier}},
  series       = {{TrAC - Trends in Analytical Chemistry}},
  title        = {{Specific monitoring of chromatographic procedures}},
  url          = {{http://dx.doi.org/10.1016/0165-9936(90)87125-6}},
  doi          = {{10.1016/0165-9936(90)87125-6}},
  volume       = {{9}},
  year         = {{1990}},
}