Specific monitoring of chromatographic procedures
(1990) In TrAC - Trends in Analytical Chemistry 9(7). p.223-227- Abstract
- On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor instrument as the detector of enzymic activity, has definite advantages. Moreover, the same instrument can be utilized in an automated, rapid on-line thermometric immunoassay for biomolecules other than enzymes.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e928c3d8-02ae-44af-8fec-baf862d5f2c0
- author
- Danielsson, B. LU and Larsson, P.-O. LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- in
- TrAC - Trends in Analytical Chemistry
- volume
- 9
- issue
- 7
- pages
- 5 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0025476988
- ISSN
- 0165-9936
- DOI
- 10.1016/0165-9936(90)87125-6
- language
- English
- LU publication?
- yes
- id
- e928c3d8-02ae-44af-8fec-baf862d5f2c0
- date added to LUP
- 2024-06-17 13:54:06
- date last changed
- 2024-08-29 12:06:01
@article{e928c3d8-02ae-44af-8fec-baf862d5f2c0, abstract = {{On-line monitoring of chromatographic procedures involving biomolecules is usually restricted to UV registration and determination of the pH or conductivity of the mobile phase. A more specific monitoring of proteins, for instance direct identification of a special enzyme, would greatly facilitate and speed up purification. We have found that flow calorimetry, performed with an enzyme thermistor instrument as the detector of enzymic activity, has definite advantages. Moreover, the same instrument can be utilized in an automated, rapid on-line thermometric immunoassay for biomolecules other than enzymes.}}, author = {{Danielsson, B. and Larsson, P.-O.}}, issn = {{0165-9936}}, language = {{eng}}, number = {{7}}, pages = {{223--227}}, publisher = {{Elsevier}}, series = {{TrAC - Trends in Analytical Chemistry}}, title = {{Specific monitoring of chromatographic procedures}}, url = {{http://dx.doi.org/10.1016/0165-9936(90)87125-6}}, doi = {{10.1016/0165-9936(90)87125-6}}, volume = {{9}}, year = {{1990}}, }