Manipulating phloem transport affects wood formation but not local nonstructural carbon reserves in an evergreen conifer
Rademacher, Tim ; Fonti, Patrick ; LeMoine, James M. ; Fonti, Marina V. ; Basler, David ; Chen, Yizhao ; Friend, Andrew D. ; Seyednasrollah, Bijan ; Eckes-Shephard, Annemarie H. LU
and Richardson, Andrew D.
(2021)
In Plant Cell and Environment
44(8).
p.2506-2521
- Abstract
How variations in carbon supply affect wood formation remains poorly understood in particular in mature forest trees. To elucidate how carbon supply affects carbon allocation and wood formation, we attempted to manipulate carbon supply to the cambial region by phloem girdling and compression during the mid- and late-growing season and measured effects on structural development, CO2 efflux and nonstructural carbon reserves in stems of mature white pines. Wood formation and stem CO2 efflux varied with a location relative to treatment (i.e., above or below the restriction). We observed up to twice as many tracheids formed above versus below the treatment after the phloem transport manipulation, whereas the cell-wall... (More)
How variations in carbon supply affect wood formation remains poorly understood in particular in mature forest trees. To elucidate how carbon supply affects carbon allocation and wood formation, we attempted to manipulate carbon supply to the cambial region by phloem girdling and compression during the mid- and late-growing season and measured effects on structural development, CO2 efflux and nonstructural carbon reserves in stems of mature white pines. Wood formation and stem CO2 efflux varied with a location relative to treatment (i.e., above or below the restriction). We observed up to twice as many tracheids formed above versus below the treatment after the phloem transport manipulation, whereas the cell-wall area decreased only slightly below the treatments, and cell size did not change relative to the control. Nonstructural carbon reserves in the xylem, needles and roots were largely unaffected by the treatments. Our results suggest that low and high carbon supply affects wood formation, primarily through a strong effect on cell proliferation, and respiration, but local nonstructural carbon concentrations appear to be maintained homeostatically. This contrasts with reports of decoupling of source activity and wood formation at the whole-tree or ecosystem level, highlighting the need to better understand organ-specific responses, within-tree feedbacks, as well as phenological and ontogenetic effects on sink-source dynamics.
(Less)
- author
- Rademacher, Tim
; Fonti, Patrick
; LeMoine, James M.
; Fonti, Marina V.
; Basler, David
; Chen, Yizhao
; Friend, Andrew D.
; Seyednasrollah, Bijan
; Eckes-Shephard, Annemarie H.
LU
and Richardson, Andrew D.
- organization
- publishing date
- 2021-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- allocation, girdling, growth, Pinus strobus, respiration, wood anatomy, xylogenesis
- in
- Plant Cell and Environment
- volume
- 44
- issue
- 8
- pages
- 16 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:34043242
- scopus:85108258914
- ISSN
- 0140-7791
- DOI
- 10.1111/pce.14117
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: A.F., A.R., T.R., and Y.C. acknowledge support from the Natural Environment Research Council (NE/P011462/1) and National Science Foundation (DEB‐1741585). A.R. is also supported by the National Science Foundation under grants DEB‐1237491 and DEB‐1832210. D.B. acknowledges support through the Swiss National Science Foundation (PSBSP3‐168701) and the Harvard Forest Bullard Fellowship. We also thank Aglaé Landry‐Boisvert, Brooklynn Abaroa, Emory Ellis, Kyle Wyche and Mark VanScoy for help in the field, Shawna Greyeyes, Amberlee Pavey and Angelina Valenzuela for help in the lab, Katharyn Duffy, Teemu Hölttä, Drew M. P. Peltier and three anonymous reviewers for feedback on the manuscript and Henrik Hartmann, Nils Henriksson, Teemu Hölttä and Cyrille Rathgeber for friendly peer‐review of the ideas and methods. Funding Information: National Science Foundation, Grant/Award Numbers: DEB‐ 1741585, DEB‐1237491, DEB‐1832210; Natural Environment Research Council, Grant/Award Number: NE/P011462/1; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Grant/Award Number: PSBSP3‐168701 Funding information Publisher Copyright: © 2021 John Wiley & Sons Ltd.
- id
- e9292b3d-71d6-42b4-b02f-c539dd8bb55a
- date added to LUP
- 2023-07-26 10:59:07
- date last changed
- 2024-04-19 23:58:10
@article{e9292b3d-71d6-42b4-b02f-c539dd8bb55a,
abstract = {{<p>How variations in carbon supply affect wood formation remains poorly understood in particular in mature forest trees. To elucidate how carbon supply affects carbon allocation and wood formation, we attempted to manipulate carbon supply to the cambial region by phloem girdling and compression during the mid- and late-growing season and measured effects on structural development, CO<sub>2</sub> efflux and nonstructural carbon reserves in stems of mature white pines. Wood formation and stem CO<sub>2</sub> efflux varied with a location relative to treatment (i.e., above or below the restriction). We observed up to twice as many tracheids formed above versus below the treatment after the phloem transport manipulation, whereas the cell-wall area decreased only slightly below the treatments, and cell size did not change relative to the control. Nonstructural carbon reserves in the xylem, needles and roots were largely unaffected by the treatments. Our results suggest that low and high carbon supply affects wood formation, primarily through a strong effect on cell proliferation, and respiration, but local nonstructural carbon concentrations appear to be maintained homeostatically. This contrasts with reports of decoupling of source activity and wood formation at the whole-tree or ecosystem level, highlighting the need to better understand organ-specific responses, within-tree feedbacks, as well as phenological and ontogenetic effects on sink-source dynamics.</p>}},
author = {{Rademacher, Tim and Fonti, Patrick and LeMoine, James M. and Fonti, Marina V. and Basler, David and Chen, Yizhao and Friend, Andrew D. and Seyednasrollah, Bijan and Eckes-Shephard, Annemarie H. and Richardson, Andrew D.}},
issn = {{0140-7791}},
keywords = {{allocation; girdling; growth; Pinus strobus; respiration; wood anatomy; xylogenesis}},
language = {{eng}},
number = {{8}},
pages = {{2506--2521}},
publisher = {{Wiley-Blackwell}},
series = {{Plant Cell and Environment}},
title = {{Manipulating phloem transport affects wood formation but not local nonstructural carbon reserves in an evergreen conifer}},
url = {{http://dx.doi.org/10.1111/pce.14117}},
doi = {{10.1111/pce.14117}},
volume = {{44}},
year = {{2021}},
}