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The lipophilic hapten parthenolide induces interferon-gamma and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro

Wahlkvist, H. ; Masjedi, K. ; Gruvberger, Birgitta LU ; Zuber, B. ; Karlberg, A. -T. ; Bruze, Magnus LU and Ahlborg, N. (2008) In British Journal of Dermatology 158(1). p.70-77
Abstract
Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an... (More)
Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an enzyme-linked immunospot (ELISpot) assay, measuring cytokine production at the single-cell level. Results The allergic group, but not the control group, responded to parthenolide with increased numbers of cells producing interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5 (P < 0.05 for all) and IL-13 (P < 0.01). The responses manifested by T-cell type 1 (IFN-gamma and IL-2) and type 2 (IL-4, IL-5 and IL-13) cytokines were positively correlated between cytokines. Subjects with a strong or moderate, but not weak or negative, patch-test reaction displayed detectable in vitro responses. In contrast to the CD4+ T cell-mediated peripheral reactivity induced by nickel, cell depletion experiments identified the parthenolide-reactive IFN-gamma- and IL-13-producing cells as CD8+ T cells. Conclusions The finding that the PBMC reactivity to parthenolide in humans involves a CD8+ T cell-mediated type 1 and 2 cytokine response warrants further studies on the relationship between the chemical nature of a hapten and the resulting immune response. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ELISpot, parthenolide, sesquiterpene lactone, cytokine, contact allergy
in
British Journal of Dermatology
volume
158
issue
1
pages
70 - 77
publisher
Wiley-Blackwell
external identifiers
  • wos:000251562900010
  • scopus:36849008570
  • pmid:17986299
ISSN
1365-2133
DOI
10.1111/j.1365-2133.2007.08287.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Occupational and Environmental Dermatology Unit (013241310), Pediatrics/Urology/Gynecology/Endocrinology (013240400)
id
e982f116-eeae-4138-8cd8-d088f6fe050a (old id 966385)
date added to LUP
2016-04-01 11:59:24
date last changed
2025-04-04 14:47:34
@article{e982f116-eeae-4138-8cd8-d088f6fe050a,
  abstract     = {{Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an enzyme-linked immunospot (ELISpot) assay, measuring cytokine production at the single-cell level. Results The allergic group, but not the control group, responded to parthenolide with increased numbers of cells producing interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5 (P &lt; 0.05 for all) and IL-13 (P &lt; 0.01). The responses manifested by T-cell type 1 (IFN-gamma and IL-2) and type 2 (IL-4, IL-5 and IL-13) cytokines were positively correlated between cytokines. Subjects with a strong or moderate, but not weak or negative, patch-test reaction displayed detectable in vitro responses. In contrast to the CD4+ T cell-mediated peripheral reactivity induced by nickel, cell depletion experiments identified the parthenolide-reactive IFN-gamma- and IL-13-producing cells as CD8+ T cells. Conclusions The finding that the PBMC reactivity to parthenolide in humans involves a CD8+ T cell-mediated type 1 and 2 cytokine response warrants further studies on the relationship between the chemical nature of a hapten and the resulting immune response.}},
  author       = {{Wahlkvist, H. and Masjedi, K. and Gruvberger, Birgitta and Zuber, B. and Karlberg, A. -T. and Bruze, Magnus and Ahlborg, N.}},
  issn         = {{1365-2133}},
  keywords     = {{ELISpot; parthenolide; sesquiterpene lactone; cytokine; contact allergy}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{70--77}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{British Journal of Dermatology}},
  title        = {{The lipophilic hapten parthenolide induces interferon-gamma and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro}},
  url          = {{http://dx.doi.org/10.1111/j.1365-2133.2007.08287.x}},
  doi          = {{10.1111/j.1365-2133.2007.08287.x}},
  volume       = {{158}},
  year         = {{2008}},
}