Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28

Hernebring, Malin ; Fredriksson, Asa ; Liljevald, Maria ; Cvijovic, Marija ; Norrman, Karin LU ; Wiseman, John ; Semb, Henrik LU and Nystrom, Thomas (2013) In Scientific Reports 3.
Abstract
In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28 alpha beta (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNF alpha. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28 alpha using miRNA counteracted the removal of damaged proteins demonstrating that PA28 alpha beta has a hitherto unidentified role required for resetting the levels of protein damage at the transition from... (More)
In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28 alpha beta (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNF alpha. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28 alpha using miRNA counteracted the removal of damaged proteins demonstrating that PA28 alpha beta has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
3
article number
1381
publisher
Nature Publishing Group
external identifiers
  • wos:000315619700003
  • scopus:84875156572
  • pmid:23459332
ISSN
2045-2322
DOI
10.1038/srep01381
language
English
LU publication?
yes
id
ea011167-46b2-4b66-aae8-c0854e90552e (old id 3669947)
date added to LUP
2016-04-01 13:02:54
date last changed
2022-04-06 02:17:27
@article{ea011167-46b2-4b66-aae8-c0854e90552e,
  abstract     = {{In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28 alpha beta (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNF alpha. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28 alpha using miRNA counteracted the removal of damaged proteins demonstrating that PA28 alpha beta has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation.}},
  author       = {{Hernebring, Malin and Fredriksson, Asa and Liljevald, Maria and Cvijovic, Marija and Norrman, Karin and Wiseman, John and Semb, Henrik and Nystrom, Thomas}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28}},
  url          = {{http://dx.doi.org/10.1038/srep01381}},
  doi          = {{10.1038/srep01381}},
  volume       = {{3}},
  year         = {{2013}},
}