Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay
(1996) In Alcohol and Alcoholism 31(4). p.397-402- Abstract
- Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated... (More)
- Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110826
- author
- Simonsson, Per LU ; Lindberg, S and Alling, Christer LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Alcohol and Alcoholism
- volume
- 31
- issue
- 4
- pages
- 397 - 402
- publisher
- Oxford University Press
- external identifiers
-
- pmid:8879289
- scopus:0029743406
- ISSN
- 1464-3502
- language
- English
- LU publication?
- yes
- id
- ea2930c2-105c-476a-825a-4b9db124d83d (old id 1110826)
- alternative location
- http://alcalc.oxfordjournals.org/cgi/reprint/31/4/397.pdf
- date added to LUP
- 2016-04-01 12:38:24
- date last changed
- 2022-01-27 07:50:28
@article{ea2930c2-105c-476a-825a-4b9db124d83d, abstract = {{Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method.}}, author = {{Simonsson, Per and Lindberg, S and Alling, Christer}}, issn = {{1464-3502}}, language = {{eng}}, number = {{4}}, pages = {{397--402}}, publisher = {{Oxford University Press}}, series = {{Alcohol and Alcoholism}}, title = {{Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay}}, url = {{http://alcalc.oxfordjournals.org/cgi/reprint/31/4/397.pdf}}, volume = {{31}}, year = {{1996}}, }