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Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality

Bhatia, Neha ; Åhl, Henrik ; Jönsson, Henrik LU and Heisler, Marcus G. (2019) In eLife 8.
Abstract

Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent... (More)

Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent findings, our results argue against the importance of auxin asymmetry in establishing leaf polarity.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
A. thaliana, auxin, developmental biology, dorsal, leaf, plant, plant biology, polarity, ventral
in
eLife
volume
8
article number
e39298
publisher
eLife Sciences Publications LTD.
external identifiers
  • scopus:85060244261
  • pmid:30667357
ISSN
2050-084X
DOI
10.7554/eLife.39298
language
English
LU publication?
yes
id
ea6c91dd-ff1d-4374-8073-8e700f9d94c7
date added to LUP
2019-01-28 13:53:44
date last changed
2020-01-22 07:25:42
@article{ea6c91dd-ff1d-4374-8073-8e700f9d94c7,
  abstract     = {<p>Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent findings, our results argue against the importance of auxin asymmetry in establishing leaf polarity.</p>},
  author       = {Bhatia, Neha and Åhl, Henrik and Jönsson, Henrik and Heisler, Marcus G.},
  issn         = {2050-084X},
  language     = {eng},
  publisher    = {eLife Sciences Publications LTD.},
  series       = {eLife},
  title        = {Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality},
  url          = {http://dx.doi.org/10.7554/eLife.39298},
  doi          = {10.7554/eLife.39298},
  volume       = {8},
  year         = {2019},
}