Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality
(2019) In eLife 8.- Abstract
Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent... (More)
Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent findings, our results argue against the importance of auxin asymmetry in establishing leaf polarity.
(Less)
- author
- Bhatia, Neha ; Åhl, Henrik ; Jönsson, Henrik LU and Heisler, Marcus G.
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- A. thaliana, auxin, developmental biology, dorsal, leaf, plant, plant biology, polarity, ventral
- in
- eLife
- volume
- 8
- article number
- e39298
- publisher
- eLife Sciences Publications
- external identifiers
-
- pmid:30667357
- scopus:85060244261
- ISSN
- 2050-084X
- DOI
- 10.7554/eLife.39298
- language
- English
- LU publication?
- yes
- id
- ea6c91dd-ff1d-4374-8073-8e700f9d94c7
- date added to LUP
- 2019-01-28 13:53:44
- date last changed
- 2024-06-11 03:24:53
@article{ea6c91dd-ff1d-4374-8073-8e700f9d94c7,
abstract = {{<p>Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent findings, our results argue against the importance of auxin asymmetry in establishing leaf polarity.</p>}},
author = {{Bhatia, Neha and Åhl, Henrik and Jönsson, Henrik and Heisler, Marcus G.}},
issn = {{2050-084X}},
keywords = {{A. thaliana; auxin; developmental biology; dorsal; leaf; plant; plant biology; polarity; ventral}},
language = {{eng}},
publisher = {{eLife Sciences Publications}},
series = {{eLife}},
title = {{Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality}},
url = {{http://dx.doi.org/10.7554/eLife.39298}},
doi = {{10.7554/eLife.39298}},
volume = {{8}},
year = {{2019}},
}