6-bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway
Zhao, Xiao E. ; Yang, Zhenshan LU
; Gao, Zhen
; Ge, Junbang
; Wei, Qing
and Ma, Baohua
(2019)
In Anais da Academia Brasileira de Ciencias
91(1).
p.1-14
- Abstract
This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured... (More)
This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM BIO exhibited detectable β-catenin expression in their nuclei, and showed upregulated β-catenin and glycogen synthase kinase 3 beta(GSK3β) phosphorylation compared to untreated cells. In addition, BIO enhanced the mRNA expression of osteoblast differentiation markers such as ALP, runt-related transcription factor 2, collagen I, osteocalcin, and osteonectin. In conclusion, BIO upregulated GSK3β phosphorylation and inhibited its activity, thereby activating the Wnt/β-catenin signaling pathway and promoting the osteogenic differentiation of canine BMSCs. The effect of 1.0 μM BIO on BMSCs differentiation was stronger than that of 0.5 μM BIO.
(Less)
- author
- Zhao, Xiao E.
; Yang, Zhenshan
LU
; Gao, Zhen
; Ge, Junbang
; Wei, Qing
and Ma, Baohua
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- keywords
- Bone mesenchymal stem cells, Bromoindirubin oxime, Canine, Osteogenetic differentiation
- in
- Anais da Academia Brasileira de Ciencias
- volume
- 91
- issue
- 1
- article number
- e20180459
- pages
- 1 - 14
- publisher
- Academia Brasileira de Ciencias
- external identifiers
-
- pmid:30916158
- scopus:85063941063
- ISSN
- 0001-3765
- DOI
- 10.1590/0001-3765201920180459
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2019, Academia Brasileira de Ciencias. All rights reserved.
- id
- eae6ee80-0d36-41f0-865a-4f4054b3acd2
- date added to LUP
- 2024-02-28 14:54:22
- date last changed
- 2024-03-01 03:35:59
@article{eae6ee80-0d36-41f0-865a-4f4054b3acd2,
abstract = {{<p>This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM BIO exhibited detectable β-catenin expression in their nuclei, and showed upregulated β-catenin and glycogen synthase kinase 3 beta(GSK3β) phosphorylation compared to untreated cells. In addition, BIO enhanced the mRNA expression of osteoblast differentiation markers such as ALP, runt-related transcription factor 2, collagen I, osteocalcin, and osteonectin. In conclusion, BIO upregulated GSK3β phosphorylation and inhibited its activity, thereby activating the Wnt/β-catenin signaling pathway and promoting the osteogenic differentiation of canine BMSCs. The effect of 1.0 μM BIO on BMSCs differentiation was stronger than that of 0.5 μM BIO.</p>}},
author = {{Zhao, Xiao E. and Yang, Zhenshan and Gao, Zhen and Ge, Junbang and Wei, Qing and Ma, Baohua}},
issn = {{0001-3765}},
keywords = {{Bone mesenchymal stem cells; Bromoindirubin oxime; Canine; Osteogenetic differentiation}},
language = {{eng}},
number = {{1}},
pages = {{1--14}},
publisher = {{Academia Brasileira de Ciencias}},
series = {{Anais da Academia Brasileira de Ciencias}},
title = {{6-bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway}},
url = {{http://dx.doi.org/10.1590/0001-3765201920180459}},
doi = {{10.1590/0001-3765201920180459}},
volume = {{91}},
year = {{2019}},
}