Activated cell cycle checkpoints in epirubicin-treated breast cancer cells studied by BrdUrd-flow cytometry
(1997) In Cytometry 29(4). p.321-327- Abstract
- Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2... (More)
- Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2 phases, presumably due to the activation of a later checkpoint. In addition, studies of the mRNA levels of p53 and its effector gene p21 revealed that although both cell lines expressed p53 mRNA, a marked difference in the mRNA levels of p21 was seen. A dramatic increase in the level of p21 mRNA was seen in epirubicin-treated MCF-7 cells, while no such increase was seen in SK-BR-3 cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111386
- author
- Hedenfalk, Ingrid LU ; Baldetorp, Bo LU ; Borg, Åke LU and Oredsson, Stina LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- breast cancer, epirubicin, p21, RNA, p53, bromodeoxyuridine, flow cytometry, cell cycle kinetics
- in
- Cytometry
- volume
- 29
- issue
- 4
- pages
- 321 - 327
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:9415415
- scopus:0031450362
- ISSN
- 0196-4763
- DOI
- 10.1002/(SICI)1097-0320(19971201)29:4<321::AID-CYTO9>3.0.CO;2-C
- language
- English
- LU publication?
- yes
- id
- eaee408b-529f-4319-87bd-219f8eeb2730 (old id 1111386)
- date added to LUP
- 2016-04-01 16:36:10
- date last changed
- 2022-01-28 20:50:27
@article{eaee408b-529f-4319-87bd-219f8eeb2730, abstract = {{Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2 phases, presumably due to the activation of a later checkpoint. In addition, studies of the mRNA levels of p53 and its effector gene p21 revealed that although both cell lines expressed p53 mRNA, a marked difference in the mRNA levels of p21 was seen. A dramatic increase in the level of p21 mRNA was seen in epirubicin-treated MCF-7 cells, while no such increase was seen in SK-BR-3 cells.}}, author = {{Hedenfalk, Ingrid and Baldetorp, Bo and Borg, Åke and Oredsson, Stina}}, issn = {{0196-4763}}, keywords = {{breast cancer; epirubicin; p21; RNA; p53; bromodeoxyuridine; flow cytometry; cell cycle kinetics}}, language = {{eng}}, number = {{4}}, pages = {{321--327}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Cytometry}}, title = {{Activated cell cycle checkpoints in epirubicin-treated breast cancer cells studied by BrdUrd-flow cytometry}}, url = {{http://dx.doi.org/10.1002/(SICI)1097-0320(19971201)29:4<321::AID-CYTO9>3.0.CO;2-C}}, doi = {{10.1002/(SICI)1097-0320(19971201)29:4<321::AID-CYTO9>3.0.CO;2-C}}, volume = {{29}}, year = {{1997}}, }