Chaperones increase association of tau protein with microtubules
Dou, Fei ; Netzer, William J. ; Tanemura, Kentaro ; Li, Feng ; Hartl, F. Ulrich ; Takashima, Akihiko ; Gouras, Gunnar K. LU
; Greengard, Paul
and Xu, Huaxi
(2003)
In Proceedings of the National Academy of Sciences of the United States of America
100(2).
p.721-726
- Abstract
Molecular chaperones and their functions in protein folding have been implicated in several neurodegenerative diseases, including Parkinson's disease and Huntington's disease, which are characterized by accumulation of protein aggregates (e.g., α-synuclein and huntingtin, respectively). These aggregates have been shown in various experimental systems to respond to changes in levels of molecular chaperones suggesting the possibility of therapeutic intervention and a role for chaperones in disease pathogenesis. It remains unclear whether chaperones also play a role in Alzheimer's disease, a neurodegenerative disorder characterized by β-amyloid and tau protein aggregates. Here, we report an inverse relationship between aggregated tau and... (More)
Molecular chaperones and their functions in protein folding have been implicated in several neurodegenerative diseases, including Parkinson's disease and Huntington's disease, which are characterized by accumulation of protein aggregates (e.g., α-synuclein and huntingtin, respectively). These aggregates have been shown in various experimental systems to respond to changes in levels of molecular chaperones suggesting the possibility of therapeutic intervention and a role for chaperones in disease pathogenesis. It remains unclear whether chaperones also play a role in Alzheimer's disease, a neurodegenerative disorder characterized by β-amyloid and tau protein aggregates. Here, we report an inverse relationship between aggregated tau and the levels of heat shock protein (Hsp)70/90 in tau transgenic mouse and Alzheimer's disease brains. In various cellular models, increased levels of Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reduce insoluble tau and cause reduced tau phosphorylation. Conversely, lowered levels of Hsp70 and Hsp90 result in the opposite effects. We have also demonstrated a direct association of the chaperones with tau proteins. Our results suggest that up-regulation of molecular chaperones may suppress formation of neurofibrillary tangles by partitioning tau into a productive folding pathway and thereby preventing tau aggregation.
(Less)
- author
- Dou, Fei
; Netzer, William J.
; Tanemura, Kentaro
; Li, Feng
; Hartl, F. Ulrich
; Takashima, Akihiko
; Gouras, Gunnar K.
LU
; Greengard, Paul
and Xu, Huaxi
- publishing date
- 2003-01-21
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 100
- issue
- 2
- pages
- 721 - 726
- publisher
- National Academy of Sciences
- external identifiers
-
- pmid:12522269
- scopus:0037457966
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.242720499
- language
- English
- LU publication?
- no
- id
- eb9c7f59-a376-48b2-bd63-aecb54f772b1
- date added to LUP
- 2020-02-20 14:26:42
- date last changed
- 2024-04-03 01:30:12
@article{eb9c7f59-a376-48b2-bd63-aecb54f772b1,
abstract = {{<p>Molecular chaperones and their functions in protein folding have been implicated in several neurodegenerative diseases, including Parkinson's disease and Huntington's disease, which are characterized by accumulation of protein aggregates (e.g., α-synuclein and huntingtin, respectively). These aggregates have been shown in various experimental systems to respond to changes in levels of molecular chaperones suggesting the possibility of therapeutic intervention and a role for chaperones in disease pathogenesis. It remains unclear whether chaperones also play a role in Alzheimer's disease, a neurodegenerative disorder characterized by β-amyloid and tau protein aggregates. Here, we report an inverse relationship between aggregated tau and the levels of heat shock protein (Hsp)70/90 in tau transgenic mouse and Alzheimer's disease brains. In various cellular models, increased levels of Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reduce insoluble tau and cause reduced tau phosphorylation. Conversely, lowered levels of Hsp70 and Hsp90 result in the opposite effects. We have also demonstrated a direct association of the chaperones with tau proteins. Our results suggest that up-regulation of molecular chaperones may suppress formation of neurofibrillary tangles by partitioning tau into a productive folding pathway and thereby preventing tau aggregation.</p>}},
author = {{Dou, Fei and Netzer, William J. and Tanemura, Kentaro and Li, Feng and Hartl, F. Ulrich and Takashima, Akihiko and Gouras, Gunnar K. and Greengard, Paul and Xu, Huaxi}},
issn = {{0027-8424}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{721--726}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences of the United States of America}},
title = {{Chaperones increase association of tau protein with microtubules}},
url = {{http://dx.doi.org/10.1073/pnas.242720499}},
doi = {{10.1073/pnas.242720499}},
volume = {{100}},
year = {{2003}},
}