Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves
(2019) In International Journal of Molecular Sciences 20(19).- Abstract
Plants have a variety of ways to defend themselves against pathogens. A commonly used model of the plant immune system is divided into a general response triggered by pathogen-associated molecular patterns (PAMPs), and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI), and the second is known as effector-triggered immunity (ETI). To obtain better insight into changes of protein abundance in immunity reactions, we performed a comparative proteomic analysis of a PTI and two different ETI models (relating to Phytophthora infestans) in potato. Several proteins showed higher abundance in all immune reactions, such as a protein annotated as sterol carrier protein 2 that could be... (More)
Plants have a variety of ways to defend themselves against pathogens. A commonly used model of the plant immune system is divided into a general response triggered by pathogen-associated molecular patterns (PAMPs), and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI), and the second is known as effector-triggered immunity (ETI). To obtain better insight into changes of protein abundance in immunity reactions, we performed a comparative proteomic analysis of a PTI and two different ETI models (relating to Phytophthora infestans) in potato. Several proteins showed higher abundance in all immune reactions, such as a protein annotated as sterol carrier protein 2 that could be interesting since Phytophthora species are sterol auxotrophs. RNA binding proteins also showed altered abundance in the different immune reactions. Furthermore, we identified some PTI-specific changes of protein abundance, such as for example, a glyoxysomal fatty acid beta-oxidation multifunctional protein and a MAR-binding protein. Interestingly, a lysine histone demethylase was decreased in PTI, and that prompted us to also analyze protein methylation in our datasets. The proteins upregulated explicitly in ETI included several catalases. Few proteins were regulated in only one of the ETI interactions. For example, histones were only downregulated in the ETI-Avr2 interaction, and a putative multiprotein bridging factor was only upregulated in the ETI-IpiO interaction. One example of a methylated protein that increased in the ETI interactions was a serine hydroxymethyltransferase.
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- author
- Resjö, Svante LU ; Zahid, Muhammad Awais ; Burra, Dharani Dhar ; Lenman, Marit LU ; Levander, Fredrik LU and Andreasson, Erik LU
- organization
- publishing date
- 2019-09-24
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Computational Biology/methods, Databases, Genetic, Mass Spectrometry, Methylation, Plant Immunity, Plant Leaves/immunology, Plant Proteins/metabolism, Protein Interaction Mapping, Proteome, Proteomics, Solanum tuberosum/immunology
- in
- International Journal of Molecular Sciences
- volume
- 20
- issue
- 19
- publisher
- MDPI AG
- external identifiers
-
- scopus:85072655307
- pmid:31554174
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms20194726
- language
- English
- LU publication?
- yes
- id
- ebade8b0-dc71-4475-a7ac-f0f02ff51129
- date added to LUP
- 2020-06-08 12:53:08
- date last changed
- 2024-05-30 17:19:37
@article{ebade8b0-dc71-4475-a7ac-f0f02ff51129,
abstract = {{<p>Plants have a variety of ways to defend themselves against pathogens. A commonly used model of the plant immune system is divided into a general response triggered by pathogen-associated molecular patterns (PAMPs), and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI), and the second is known as effector-triggered immunity (ETI). To obtain better insight into changes of protein abundance in immunity reactions, we performed a comparative proteomic analysis of a PTI and two different ETI models (relating to Phytophthora infestans) in potato. Several proteins showed higher abundance in all immune reactions, such as a protein annotated as sterol carrier protein 2 that could be interesting since Phytophthora species are sterol auxotrophs. RNA binding proteins also showed altered abundance in the different immune reactions. Furthermore, we identified some PTI-specific changes of protein abundance, such as for example, a glyoxysomal fatty acid beta-oxidation multifunctional protein and a MAR-binding protein. Interestingly, a lysine histone demethylase was decreased in PTI, and that prompted us to also analyze protein methylation in our datasets. The proteins upregulated explicitly in ETI included several catalases. Few proteins were regulated in only one of the ETI interactions. For example, histones were only downregulated in the ETI-Avr2 interaction, and a putative multiprotein bridging factor was only upregulated in the ETI-IpiO interaction. One example of a methylated protein that increased in the ETI interactions was a serine hydroxymethyltransferase.</p>}},
author = {{Resjö, Svante and Zahid, Muhammad Awais and Burra, Dharani Dhar and Lenman, Marit and Levander, Fredrik and Andreasson, Erik}},
issn = {{1422-0067}},
keywords = {{Computational Biology/methods; Databases, Genetic; Mass Spectrometry; Methylation; Plant Immunity; Plant Leaves/immunology; Plant Proteins/metabolism; Protein Interaction Mapping; Proteome; Proteomics; Solanum tuberosum/immunology}},
language = {{eng}},
month = {{09}},
number = {{19}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves}},
url = {{http://dx.doi.org/10.3390/ijms20194726}},
doi = {{10.3390/ijms20194726}},
volume = {{20}},
year = {{2019}},
}