Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer
Hall, Stephen C.L. ; Clifton, Luke A. ; Tognoloni, Cecilia ; Morrison, Kerrie A. ; Knowles, Timothy J. ; Kinane, Christian J. ; Dafforn, Tim R. ; Edler, Karen J. LU
and Arnold, Thomas
(2020)
In Journal of Colloid and Interface Science
574.
p.272-284
- Abstract
Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate... (More)
Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins.
(Less)
- author
- Hall, Stephen C.L.
; Clifton, Luke A.
; Tognoloni, Cecilia
; Morrison, Kerrie A.
; Knowles, Timothy J.
; Kinane, Christian J.
; Dafforn, Tim R.
; Edler, Karen J.
LU
and Arnold, Thomas
- publishing date
- 2020-08-15
- type
- Contribution to journal
- publication status
- published
- keywords
- Adsorption, Lipid exchange, Neutron reflectometry, Polymer-stabilized phospholipid nanodisc, styrene maleic acid (SMA), styrene-maleic acid lipid particle (SMALP), Supported lipid bilayer
- in
- Journal of Colloid and Interface Science
- volume
- 574
- pages
- 13 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:32330753
- scopus:85083424047
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2020.04.013
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2020
- id
- ebc51a0f-161f-4769-87c6-432e9fe99f2d
- date added to LUP
- 2023-01-18 09:01:00
- date last changed
- 2024-04-18 10:03:44
@article{ebc51a0f-161f-4769-87c6-432e9fe99f2d,
abstract = {{<p>Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins.</p>}},
author = {{Hall, Stephen C.L. and Clifton, Luke A. and Tognoloni, Cecilia and Morrison, Kerrie A. and Knowles, Timothy J. and Kinane, Christian J. and Dafforn, Tim R. and Edler, Karen J. and Arnold, Thomas}},
issn = {{0021-9797}},
keywords = {{Adsorption; Lipid exchange; Neutron reflectometry; Polymer-stabilized phospholipid nanodisc; styrene maleic acid (SMA); styrene-maleic acid lipid particle (SMALP); Supported lipid bilayer}},
language = {{eng}},
month = {{08}},
pages = {{272--284}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer}},
url = {{http://dx.doi.org/10.1016/j.jcis.2020.04.013}},
doi = {{10.1016/j.jcis.2020.04.013}},
volume = {{574}},
year = {{2020}},
}