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Differential regulation of α and β chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S

Garcia de Frutos, Pablo LU ; Alim, R.I.M.; Härdig, Ylva; Zöller, Bengt LU and Dahlbäck, Björn LU (1994) In Blood 84(3). p.815-822
Abstract
Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for β chain containing C4BP (C4BPβ+) was developed and the concentrations of total C4BP, C4BPβ+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BPβ+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BPβ+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BPβ+. However, in the acute-phase group, the correlation between... (More)
Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for β chain containing C4BP (C4BPβ+) was developed and the concentrations of total C4BP, C4BPβ+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BPβ+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BPβ+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BPβ+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BPβ+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP α- and β-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BPβ+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
binding protein, complement component C4b, protein S, acute phase response, alpha chain, article, beta chain, human, plasma, priority journal, protein blood level, protein determination, serum
in
Blood
volume
84
issue
3
pages
8 pages
publisher
American Society of Hematology
ISSN
1528-0020
language
English
LU publication?
yes
id
ec14e5d6-6fe2-4497-af6e-e1a32fd4101c
date added to LUP
2017-11-02 11:26:04
date last changed
2017-11-08 08:04:55
@article{ec14e5d6-6fe2-4497-af6e-e1a32fd4101c,
  abstract     = {Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for β chain containing C4BP (C4BPβ+) was developed and the concentrations of total C4BP, C4BPβ+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BPβ+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BPβ+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BPβ+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BPβ+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP α- and β-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BPβ+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.},
  author       = {Garcia de Frutos, Pablo and Alim, R.I.M. and Härdig, Ylva and Zöller, Bengt and Dahlbäck, Björn},
  issn         = {1528-0020},
  keyword      = {binding protein,complement component C4b,protein S,acute phase response,alpha chain,article,beta chain,human,plasma,priority journal,protein blood level,protein determination,serum},
  language     = {eng},
  month        = {11},
  number       = {3},
  pages        = {815--822},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Differential regulation of α and β chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S},
  volume       = {84},
  year         = {1994},
}