The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates.
(2009) In Biotechnology and Bioengineering 104. p.698-708- Abstract
- Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen... (More)
- Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen pool stored in the SBR cycle. Under aerobic and ammonia limited conditions, PHA comprising only 3HB was accumulated to 60 % of cell dry weight. Glycogen was consumed during aerobic PHA accumulation as well as under anaerobic conditions, but with different stoichiometry. Under aerobic conditions 0.31 C-mol glycogen was consumed per consumed C-mol acetate compared to 0.99 under anaerobic conditions. Both the PHA biomass content and the specific PHA production rate obtained were similar to what is typically obtained using the more commonly applied aerobic dynamic feeding strategy. (c) 2009 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1434243
- author
- Bengtsson, Simon LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 104
- pages
- 698 - 708
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000271173400007
- pmid:19530079
- scopus:70350492881
- pmid:19530079
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.22444
- language
- English
- LU publication?
- yes
- id
- ec2c1a2d-860b-4670-8e37-a1347916c97d (old id 1434243)
- date added to LUP
- 2016-04-01 12:26:34
- date last changed
- 2022-04-21 07:32:20
@article{ec2c1a2d-860b-4670-8e37-a1347916c97d,
abstract = {{Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen pool stored in the SBR cycle. Under aerobic and ammonia limited conditions, PHA comprising only 3HB was accumulated to 60 % of cell dry weight. Glycogen was consumed during aerobic PHA accumulation as well as under anaerobic conditions, but with different stoichiometry. Under aerobic conditions 0.31 C-mol glycogen was consumed per consumed C-mol acetate compared to 0.99 under anaerobic conditions. Both the PHA biomass content and the specific PHA production rate obtained were similar to what is typically obtained using the more commonly applied aerobic dynamic feeding strategy. (c) 2009 Wiley Periodicals, Inc.}},
author = {{Bengtsson, Simon}},
issn = {{1097-0290}},
language = {{eng}},
pages = {{698--708}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates.}},
url = {{http://dx.doi.org/10.1002/bit.22444}},
doi = {{10.1002/bit.22444}},
volume = {{104}},
year = {{2009}},
}